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提高榨菜离体培养植株再生频率
引用本文:余小林,曹家树,陈石头,吴剑丙.提高榨菜离体培养植株再生频率[J].细胞生物学杂志,2004,26(4):439-443.
作者姓名:余小林  曹家树  陈石头  吴剑丙
作者单位:浙江大学蔬菜研究所,浙江大学蔬菜研究所,浙江大学蔬菜研究所,浙江大学蔬菜研究所 农业部园艺植物生长发育与生物技术重点开放实验室,杭州 310029,农业部园艺植物生长发育与生物技术重点开放实验室,杭州 310029,农业部园艺植物生长发育与生物技术重点开放实验室,杭州 310029,农业部园艺植物生长发育与生物技术重点开放实验室,杭州 310029
基金项目:浙江省重大科技项目资助(No.021102536)~~
摘    要:采用榨菜“浙桐1号”品种为材料,以MS为基本培养基,通过对不同植物生长调节剂的组合和不同外植体等主要因素的筛选,大幅度提高了榨菜离体培养植株再生频率。结果表明,2mg/L6.BA 0.2mg/L2,4-D的组合较为适宜,其不定芽再生频率可达50%,且外植体以下胚轴为好:而CPPU和2,4-D的适宜组合为1.5mg/L 0.2mg/L,其不定芽再生频率高达66.67%,最适外植体为带柄子叶。同时,研究结果显示,添加0.25~1mg/L的GA,对榨菜已分化的不定芽的伸长有抑制作用;子叶柄和下胚轴外植体的分化具有极性现象。

关 键 词:榨菜  组织培养  植株再生  器官发生  外植体
修稿时间:2003年12月31

Improvement of Plant Regeneration from Mustard (Brassica juncea var.tumida) Cultured in vitro
Xiao-Lin Yu,Jia-Shu Cao,Shi-Tou Chen,Jian-Bing Wu.Improvement of Plant Regeneration from Mustard (Brassica juncea var.tumida) Cultured in vitro[J].Chinese Journal of Cell Biology,2004,26(4):439-443.
Authors:Xiao-Lin Yu  Jia-Shu Cao  Shi-Tou Chen  Jian-Bing Wu
Abstract:An efficient regeneration system of mustard (Brassica juncea Czern. et Coss. var. tumida Tsen et Lee cv. Zhetong No. 1) was developed in this study. The results showed that regeneration ratio was fifty percent, when supplemented with 2 mg/L 6-BA and 0.2 mg/L 2,4-D on Murashige and Skoog (MS) medium and hypocotyl as explant; while the regeneration ratio was sixty seven percent, when supplemented with 1.5 mg/L CPPU and 0.2 mg/L 2,4-D on MS medium, and the optimal explant was cotyledon with petiole. Moreover, the result indicated that CPPU were more effective in callus induction and shoot regeneration. Interestingly, the elongation of regenerated buds was inhibited when supplemented with 0.25-1 mg/L GA3, and the phenomenon of polarity was observed in regeneration of cotyledon petiole and hypocotyl explants. This may help in the gene transformation to improve agronomic characters in mustard for breeding programmes.
Keywords:mustard (Brassica juncea var  tumida)  tissue culture  plant regeneration  organogenesis
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