| 蛋白磷酸酯酶-1和-2A抑制剂对NG108-15细胞tau蛋白磷酸化的影响 |
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| 引用本文： | 朱粹青,曹小定.蛋白磷酸酯酶-1和-2A抑制剂对NG108-15细胞tau蛋白磷酸化的影响[J].细胞生物学杂志,2002,24(2):115-117. |
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| 作者姓名： | 朱粹青 曹小定 |
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| 作者单位： | 复旦大学医学院医学神经生物国家重点实验室，上海200032 |
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| 基金项目： | 国家自然科学基金39830450，国家重点基础研究基金G1999054007，教技司 2000—66 |
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| 摘 要： | Alzheimer痴呆(AD)的主要脑病理变化之一为由超磷酸化的tau蛋白组成的神经原纤维缠结(Neurofibrillary tangle,NFT)。AD的Tau蛋白异常磷酸化与蛋白磷酸酯酶(PP)-2A和-1缺陷有关。本文首先用免疫印迹法显示NG含两大组分的tau蛋白。MTT方法观察到PP-2A和PP-1抑制剂冈 田酸(Okadaic acid.OA)处理NG108-14细胞能导致细胞代谢明显下降,同时免疫印迹法显示OA能导致的NG细胞Tau蛋白磷酸化。该研究为建立AD样蛋白磷酸酯酶缺陷引起的tau蛋白磷酸化的细胞模型奠定了基础。
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| 关 键 词： | AB PP－2A抑制剂 蛋白磷酸酯酶－1 抑制剂 NG108－15细胞 tau蛋白磷酸化 |
| 修稿时间： | 2000年9月25日 |
EFFECT OF PROTEIN PHOSPHATASE-1 AND 2A INHIBITOR ON TAU PROTEIN PHOSPHORYLATION OF NG108-14 CELL |
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| ZHU Cui Qing CAO Xiao Ding..EFFECT OF PROTEIN PHOSPHATASE-1 AND 2A INHIBITOR ON TAU PROTEIN PHOSPHORYLATION OF NG108-14 CELL[J].Chinese Journal of Cell Biology,2002,24(2):115-117. |
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| Authors: | ZHU Cui Qing CAO Xiao Ding |
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| Abstract: | One of Alzheimer dementia( AD) pathological hallmark in brain is the large amount of neurofibrillary tangles which are made by hy-perphosphorylated tau protein. The deficiency of protein phosphatases(PP)-1 and -2A is involved in abnormal phosphorylation of tau of AD cases. In this study, tau protein of NG108-14 cells was analyzed by western blot. After treated with PP-1 and PP-2A specific in-hibitor-okadaic acid(OA), reduction of MTT of NG108-14 decreased as well as the elevating phosphorylated tau protein was showed by western blots. This study has provided the AD like tau phosphoylation cell model for our further research. |
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| Keywords: | Alzheimer disease Okadaic acid Tau protein |
| 本文献已被 CNKI 维普 等数据库收录！ |
