携带TRAIL的溶瘤腺病毒联合LiCl对癌细胞的生长抑制效应

研究糖原合成激酶3的抑制剂LiCl联合携带TRA／L基因的溶瘤腺病毒Ad．sp—ElA—E1B（△55kDa）．TRAIL—Flag（Ad．sp—TRAIL—Flag）对癌细胞的体外杀伤作用。采用MTT法检测癌症特异性病毒Ad．sp—TRAIL—Flag联合药物LiCl对三种癌症细胞株的生长抑制作用；通过结晶紫实验进一步检测联合用药的杀伤效果：进而通过Western blot实验检测联合作用对癌细胞中TRAIL蛋白表达的影响，最后通过流式细胞仪检测其对癌细胞的凋亡作用。结果显示，LiCl联合Ad．sp—TRAIL．Flag的处理对癌细胞的增殖抑制作用明显优于两者单独使用。Western blot实验证明，LiCl可提高溶瘤腺病毒Ad．sp—TRAIL—FIag处理后TRAIL蛋白的表达水平，从而增强了溶瘤腺病毒Ad．sp—TRAIL—Flag通过乃己4儿的信号通路的杀伤效果。

The Growth Inhibiting Effect of Oncolytic Adenovicus-Mediated TRAIL Combined with LiCl on Cancer Cells

We investigated the killing effect of glycogen synthesis kinase 3 inhibitor LiC1 combained with Ad.sp-ElA-ElB（A55 kDa）TRAIL-Flag （also known as Ad.sp-TRAIL-Flag） in vitro that oncolytic adenovirus carries on human tumor ceils. MTT assay is used to detect inhibition on tumor cells proliferation by drug LiCl combined with tumor-specific virus Ad.sp-TRAIL-Flag, and combination effect on medication cytotoxicity are further detected by crystal violet experiment; Moreover, Western blot assays are performed to test the expression change of TRAIL. In addition, the apoptosis of these cancer cells was inspected by flow cytometry. The result shows that the growth inhibiting effect of LiCl combined with Ad.sp-TRAIL-Flag treatment on cancer cells is better than using them separately, so as to enhance the express of TRAIL through the signal path killing effects.