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家兔供体细胞的发育周期与重构胚发育的关系
引用本文:郝茹,王玉阁,张正旺,杜淼.家兔供体细胞的发育周期与重构胚发育的关系[J].细胞生物学杂志,2004,26(6):631-634.
作者姓名:郝茹  王玉阁  张正旺  杜淼
作者单位:[1]北京师范大学生命科学学院,北京100875 [2]中国科学院遗传与发育生物学研究所,北京100080
基金项目:国家重点基础研究发展项月(973计划)(No.2000016107) 中国科学院知识创新项目(No.KSCX2-SW-303)资助
摘    要:采用血清饥饿法处理体外培养的兔子胎儿成纤维细胞,并将其作为供体细胞移入去核卵母细胞内构建重构胚胎。检查供体细胞的细胞周期对重构胚的融合率、分裂率和着床率的影响。实验结果表明:培养基中血清含量在0.5%的情况下,G0/G1期的细胞比例由正常培养条件下(培乔液中含有10%FCS)的73.2%明显地增加到86%以上。饥饿1~3天的细胞作为供体细胞构建重构胚时,可明显提高重构胚的融合率,但是不同的饥饿时间其融合率并无显著的差异。饥饿处理可明显增加重构胚的分裂率,以饥饿处理3天为最佳。

关 键 词:家兔  供体细胞  发育周期  重构胚发育  血清饥饿法  胎儿成纤维细胞  体细胞核移植
修稿时间:2004年4月22日

The Relationship of the Rabbit Somatic Donor Cells at Different Cell Cycle and the Development of Nuclear-transferred Embryos
Ru Hao,Yu-Ge Wang,Zheng-Wang Zhang,Miao Du.The Relationship of the Rabbit Somatic Donor Cells at Different Cell Cycle and the Development of Nuclear-transferred Embryos[J].Chinese Journal of Cell Biology,2004,26(6):631-634.
Authors:Ru Hao  Yu-Ge Wang  Zheng-Wang Zhang  Miao Du
Abstract:Cell cycle of rabbit fetal fibroblast cells treated with or without serum starvation in vitro was analyzed by flow cytometer and nuclear-transferred embryos were examined in vitro and in vivo. Serum starvation could remarkably increase the percentages of G0/G1 stages from 73.2% in normal condition (containing 10% PCS) to above 86%, but there were no significant difference among serum starvation for 1,2 and 3 days. Donor cells treated with different culture time were fused into enucleated oocytes. And the fusion rate between donor cell and enucleated oocyte, division rate of reconstructed embryos cultured in vitro and implantation rate of reconstructed embryos in vivo were compared, in which showed that: serum starvation could significantly increase the fusion rate, division rate and embryo implantation rate; donor cells starved for 1 day or more could largely increase the fusion rate and division percentage, starved for 1 days could increase the reconstructed embryo implantation rate in vivo.
Keywords:fetal fibroblast cells  somatic nuclear transfer  donor cells  serum starvation  rabbit  
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