The cytogenetic and hepatotoxic effects of dioxin on mouse liver cells |
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Authors: | Antone L Brooks Scott W Jordan Kallol K Bose Jennifer Smith David C Allison |
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Institution: | (1) Lovelace Inhalation Toxicology Research Institute, Albuquerque, New Mexico;(2) Department of Pathology, The University of New Mexico School of Medicine, Albuquerque, New Mexico;(3) Veterans Administration Medical Center, Albuquerque, New Mexico;(4) Assaigai Laboratories, Albuquerque, New Mexico;(5) Lovelace Inhalation Toxicology Research Institute, P.O. Box 5890, 87185 Albuquerque, NM;(6) Present address: Department of Surgery, Baltimore Veterans Administration Medical Center and Johns Hopkins University School of Medicine, 21205 Baltimore, MD |
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Abstract: | The cytogenetic and hepatotoxic effects of 2,3,7,8-tetrachlorodibenzo p-dioxin (TCDD) on mouse liver cells were investigated. Male C57BL/6J strain mice, which have TCDD receptors, were given single intraperitoneal injections of 25, 37.5, 75 and 150 g of TCDD/kg body weight or corn oil carrier alone. Two-thirds hepatectomies were carried out at 1 or 7 days after injection and chromosomal aberrations and mitotic indexes of the regenerating hepatocytes were scored 54 hr after hepatectomy. Liver sections from additional intact mice were studied for TCDD-hepatotoxicity at 1, 7 and 30 days after injection. The three high doses of TCDD caused hepatotoxicity with necrosis of liver cells and focal architectural collapse by 30 days after injection. No evidence was obtained of an increase in the frequency of chromosomal structural aberrations at doses that allowed sufficient mitotic activity for cytogenetic evaluation. We conclude that TCDD is not a clastogen for mouse hepatocytes, although high doses cause marked hepatocellular necrosis.Abbreviations CSD
chromosome deletion
- META
metacentric chromosome
- TCDD
2,3,7,8-tetrachlorobenzo-p-dioxin |
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Keywords: | chromosome aberrations dioxin hepatotoxicity liver |
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