Culturing precision-cut human prostate slices as an in vitro model of prostate pathobiology |
| |
Authors: | Parrish A R Sallam K Nyman D W Orozco J Cress A E Dalkin B L Nagle R B Gandolfi A J |
| |
Institution: | (1) Department of Medical Pharmacology, Texas A&M University, College Station, Texas, USA;(2) Department of Pathology, College of Medicine, University of Arizona, Tucson, Arizona, USA;(3) Department of Surgery, College of Medicine, University of Arizona, Tucson, Arizona, USA |
| |
Abstract: | Due to the complex morphology of the prostate, it was hypothesized that precision-cut tissue slices from human prostate would
provide a unique in vitro model. Precision-cut slices were generated from zones of human prostate and their viability was assessed under conditions
of different media for up to 120 h. Slices were also exposed to several concentrations of CdCl2, which was used as a model toxicant. Maintenance of both stromal and epithelial cells was noted; however, there was a gradual
loss of luminal epithelial cells when the medium was not supplemented with dihydrotestosterone (DHT). Minimal leakage of lactate
dehydrogenase occurred throughout the incubation. Prostate-specific antigen (PSA) was detected in the medium at all time points,
although the rates of secretion fell over time. There was a loss of PSA-positive cells when the medium was not supplemented
with DHT, consistent with a loss of luminal cells, whereas PSA-positive cells were maintained in the DHT-supplemented media.
A proliferation of basal cells was observed in the presence of media containing 10% fetal bovine serum. Exposure of slices
to CdCl2 demonstrated a dose-response effect ranging from proliferation to complete cellular necrosis. Given the retention of stromal-epithelial
interactions and the use of acquired human tissue, prostate slices represent a unique in vitro model for investigating human prostate pathobiology.
This revised version was published online in July 2006 with corrections to the Cover Date. |
| |
Keywords: | prostate dynamic organ culture system peripheral transitional stromal– epithelial |
本文献已被 PubMed SpringerLink 等数据库收录! |
|