Estrogen-like properties of brominated analogs of bisphenol A in the MCF-7 human breast cancer cell line |
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Authors: | M Samuelsen C Olsen JA Holme E Meussen-Elholm A Bergmann JK Hongslo |
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Institution: | (1) Department of Environmental Medicine, National Institute of Public Health, Torshov, Oslo, Norway;(2) University of Stockholm, Sweden |
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Abstract: | Tetrabromobisphenol A (TeBBPA) is a four-meta-brominated variant of bisphenol A (BPA) and is one of the most commonly used brominated flame retardants worldwide. We compared
the estrogenic potency of TeBBPA, BPA and the brominated analogs mono- (MBBPA), di- (DBBPA), and tribromobisphenol A (TrBBPA)
in the estrogen-dependent human breast cancer cell line MCF-7. All of the compounds competed with 17β-estradiol for binding
to the estrogen receptor, although the affinity of the test chemicals to the estrogen receptor was much lower than that of
17β-estradiol. TrBBPA and TeBBPA showed a considerably lower access to the estrogen receptors within intact MCF-7 cells incubated
in 100% serum compared to incubation in serum-free medium, indicating a strong binding to serum proteins. BPA, MBBPA, and
DBBPA showed only a slightly reduced access to the receptors. All of the test compounds induced proliferation in MCF-7 cells,
the potential decreasing with increasing number of bromo-substitutions. TeBBPA did not induce maximal cell growth, indicating
cytotoxic effects at high concentrations. BPA and the brominated analogs, except TeBBPA, induced progesterone receptor and
pS2 to the same extent as 17β-estradiol, although at much higher concentrations. Our studies demonstrate that compared to
17β-estradiol, BPA and the brominated analogs have much lower estrogenic potencies for all of the endpoints tested, TeBBPA
being the least estrogenic compound.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | bisphenol A estrogen-like effects MCF-7 cells structure– activity relationship tetrabromobisphenol A |
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