Identification of novel genes putatively involved in the photosystem synthesis of <Emphasis Type="Italic">Bradyrhizobium</Emphasis> sp. ORS 278 |
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Authors: | Marianne Jaubert Laure Hannibal Joël Fardoux Eric Giraud André Verméglio |
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Institution: | (1) Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD, CIRAD, AGRO-M, INRA, UM2. TA A-82/J, Campus de Baillarguet, 34398 Montpellier Cedex 5, France;(2) CEA, DSV, IBEB, Lab Bioenerget Cellulaire, 13108 Saint-Paul-lez-Durance, France;(3) CNRS, UMR Biol Veget & Microbiol Environ, 13108 Saint-Paul-lez-Durance, France;(4) Aix-Marseille Université, 13108 Saint-Paul-lez-Durance, France |
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Abstract: | In aerobic anoxygenic phototrophs, oxygen is required for both the formation of the photosynthetic apparatus and an efficient
cyclic electron transfer. Mutants of Bradyrhizobium sp. ORS278 affected in photosystem synthesis were selected by a bacteriochlorophyll fluorescence-based screening. Out of
the 9,600 mutants of a random Tn5 insertion library, 50 clones, corresponding to insertions in 28 different genes, present
a difference in fluorescence intensity compared to the WT. Besides enzymes and regulators known to be involved in photosystem
synthesis, 14 novel components of the photosynthesis control are identified. Among them, two genes, hsIU and hsIV, encode components of a protein degradation complex, probably linked to the renewal of photosystem, an important issue in
Bradyrhizobia which have to deal with harmful reactive oxygen species. The presence of homologs in non-photosynthetic bacteria for most
of the regulatory genes identified during study suggests that they could be global regulators, as the RegA–RegB system.
Eric Giraud and André Verméglio should be considered co-senior authors. |
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Keywords: | Photosystem regulation Aerobic photosynthetic bacteria Bradyrhizobium |
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