Fluorescence studies on interaction between phospho-LHC II and subchloroplast Photosystem 1 preparations |
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Authors: | Svetlana M Kochubey Victor V Shevchenko Olga I Volovik |
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Institution: | (1) Institute of Plant Physiology and Genetics, Ukrainian Academy of Sciences, 252022 Kiev, Ukraine |
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Abstract: | Chloroplast proteins were phosphorylated under two test conditions: white light irradiance alone and white light irradiance with the addition of glucose and glucose oxidase, used to produce an anaerobic medium. The interaction of phospho-LHC II with Photosystem 1 (PS 1) was studied for two types of PS I preparation. Changes in the chlorophyll a/b ratio and the ratio of 650 and 680 nm band intensities (E650/E680) in fluorescence excitation spectra were used in calculating the phospho-LHC II portion which became associated with PS 1. It is shown that the associated portion of phospho-LHC II varies for each of the PS 1 preparations and phosphorylation procedures. Possible conclusions as regards the transfer of various sets of LHC II subpopulations under different phosphorylation procedures and the differences of interaction with PS 1 are discussed.Abbreviations PS 1
Photosystem 1
- PS 2
Photosystem 2
- LHC II
light-harvesting chlorophyll a/b protein complex II
- Chl
chlorophyll
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fluorescence quantum yield
- f
life time of fluorescence at =685 nm
- F735
fluorescence band with a maximum at 735 nm
- F685
fluorescence band with a maximum at 685 nm
- E650/E680
ratio of amplitudes in excitation fluorescence spectrum at 650 and 680 nm |
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Keywords: | light-harvesting chlorophyll a/b protein complex II phosphorylation of chloroplast membrane proteins low temperature fluorescence spectra fluorescence excitation spectra |
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