The quantum efficiency of photosystem II and its relation to non-photochemical quenching of chlorophyll fluorescence; the effect of measuring-and growth temperature |
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Authors: | Klaas J van Wijk Philip R van Hasselt |
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Institution: | (1) Department of Plant Biology, University of Groningen, P.O. Box 14, 9750 AA Haren, The Netherlands |
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Abstract: | The relation between the quantum yield of oxygen evolution of open photosystem II reactions centers (p), calculated according to Weis and Berry (1987), and non-photochemical quenching of chlorophyll fluorescence of plants grown at 19°C and 7°C was measured at 19°C and 7°C. The relation was linear when measured at 19°C, but when measured at 7°C a deviation from linearity was observed at high values of non-photochemical quenching. In plants grown at 7°C this deviation occurred at higher values of non-photochemical quenching than in plants grown at 19°C. The deviations at high light intensity and low temperature are ascribed to an increase in an inhibition-related, non-photochemical quenching component (qI).The relation between the quantum yield of excitation capture of open photosystem II reaction centers (exe), calculated according to Genty et al. (1989), and non-photochemical quenching of chlorophyll fluorescence was found to be non-linear and was neither influenced by growth temperature nor by measuring temperature.At high PFD the efficiency of overall steady state electron transport measured by oxygen-evolution, correlated well with the product of q
N
and the efficiency of excitation capture (exe) but it deviated at low PFD. The deviations at low light intensity are attributed to the different populations of chloroplasts measured by gas exchange and chlorophyll fluorescence and to the light gradient within the leaf.Abbreviations F0
basic fluorescence
- F0
basic fluorescence, thylakoid in energized state
- Fm
maximal fluorescence
- Fm
maximum fluorescence in energized state
- Fs
steady state fluorescence
- Fv
maximal variable fluorescence
- PFD
photon flux density
- PS IIrc
Photosystem II reaction center
- qF0
quenching of basic fluorescence
- qE
energy related quenching
- qN
non-photochemical quenching:-qf-total quenching
- qI
inhibition-related quenching
- qp
photochemical quenching
- qr
quenching due to state transition
- Rd
dark respiration
- p
PS II efficiency of excitation capture of open PS IIrc
- pe
extrapolated minimal value of p
- p0
extrapolated maximal value of p
- si
quantum efficiency of linear electron transport, calculated from gas exchange measurements based on incident light
- sf
quantum efficiency of linear electron transport, calculated from fluorescence measurements, based on incident measuring light |
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Keywords: | electron transport low temperature oxygen-evolution quantum yield Spinacia oleracea cv estivato |
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