Discrimination between <Emphasis Type="Italic">Haemaphysalis longicornis</Emphasis> and <Emphasis Type="Italic">H. qinghaiensis</Emphasis> based on the partial 16S rDNA and the second internal transcribed spacer (ITS-2) |
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Authors: | Zhancheng Tian Guangyuan Liu Junren Xie Hong Yin Jianxun Luo Liyan Zhang Ping Zhang Jin Luo |
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Institution: | (1) Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 Xujianping, 730046 Yanchangbu, Lanzhou, Gansu Province, People’s Republic of China; |
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Abstract: | In the present study, two hard tick species, Haemaphysalis longicornis and H. qinghaiensis from North-western China were characterized genetically by the second internal transcribed spacer (ITS-2) of nuclear ribosomal
DNA and partial 16S rDNA. Based on a fragment within the hypervariable region of 16S rDNA with the length of approximately
453 bp, the phylogenetic trees were constructed by Neighbor-Joining and Maximum-parsimony methods. The results indicated that
the phylogenetic status of H. qinghaiensis was distant from that of H. longicornis and closer to H. flava. Furthermore, the ITS-2 rDNA was amplified by PCR and sequenced from individual ticks. The length of ITS-2 is 1,606 bp for
H. longicornis and 1,162 bp for H. qinghaiensis. Although sequence variation between the immature stages of H. longicornis was 0.1–0.4%, nucleotide differences between the tested species ranged 2.1–23.2%, indicating that ITS-2 rDNA sequences are
genetic markers for the differentiation of the two hard ticks in China. Hence, a PCR-linked restriction fragment length polymorphism
(RFLP) approach was developed for their unequivocal differentiation based on ITS-2 rDNA, which provides the foundation for
further studies on ticks in China and has implications for studying the population genetic structure of the ticks and for
identification and differentiation of closely related ticks. |
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