PCR-RFLP genotyping for Japanese and Korean populations of Pacific oyster using mitochondrial DNA noncoding region |
| |
Authors: | Takane Okimoto Kenji Hara Tadashi Ishihara Futoshi Aranishi |
| |
Institution: | (1) Graduate School of Science and Technology, Nagasaki University, Nagasaki 852-8521, Japan;(2) Department of Biological and Environmental Sciences, Miyazaki University, Miyazaki 889-2192, Japan |
| |
Abstract: | Production of Pacific oyster Crassostrea gigas in Miyagi and Hiroshima (Japan) has gradually increased, with a marked increase in imported oysters from Goseong (Korea),
and then cultured oysters of the Miyagi, Hiroshima, and Goseong populations accounting for most oyster consumption in Japan.
In this study, we developed a simple PCR-RFLP analysis of a mitochondrial DNA noncoding region (mtDNA-NCR) for differentiating
cultured oysters of these populations. PCR amplification yielded a 818 bp fragment comprising the entire mtDNA-NCR and parts
of adjacent tRNACys and tRNAAsn genes from all the specimens. By use of a wide restriction test using 30 different restriction enzymes, only a single enzyme
only, AluI, produced a unique RFLP pattern enabling us to discriminate Miyagi and Hiroshima oysters from Goseong oysters. This difference
is probably because of nucleotide alteration at the presumptive AluI recognizing site on position 439 of the mtDNA-NCR. Our simple, robust and cost-effective PCR-RFLP analysis is potentially
useful for population genetic investigation of cultured Pacific oyster, particularly when large numbers of specimens must
be analyzed. |
| |
Keywords: | Pacific oyster PCR-RFLP Mitochondrial DNA Noncoding region Cultured population |
本文献已被 SpringerLink 等数据库收录! |
|