HPLC-CD selectivity assay for alcohol dehydrogenases |
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Authors: | Hamzic Melissa Pietruszka Jörg Sandkuhl Diana |
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Institution: | Institut für Bioorganische Chemie Heinrich-Heine-Universit?t Düsseldorf im Forschungszentrum, Jülich, Stetternicher Forst, Geb. 15.8, D-52426 Jülich, Germany. |
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Abstract: | Enantioselective reductions are a key to successful target-oriented syntheses. Finding the most suitable conditions is often a tedious work that is especially hampered by the time-consuming analytical investigation. A possible solution is the combined use of high-performance liquid chromatography and circular dichroism to find a suitable system for providing enantiomerically pure alcohols. This investigation led to an efficient protocol for the alcohol dehydrogenase-catalyzed reduction of 1-phenyl-2-propyn-3-trimethylsilyl-1-on (1). |
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