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| 生防菌ZJY-1及ZJY-116的GFP标记及其在黄瓜根围的生态适应性 | |
| 引用本文： | 张昕,张炳欣,喻景权,张震,沈卫峰,陈振宇,石江.生防菌ZJY-1及ZJY-116的GFP标记及其在黄瓜根围的生态适应性[J].应用生态学报,2005,16(11):2144-2148. |
| 作者姓名： | 张昕 张炳欣 喻景权 张震 沈卫峰 陈振宇 石江 |
| 作者单位： | 1. 浙江大学农业与生物技术学院,杭州,310029 2. 浙江农业科学院,杭州,310021 3. 浙江大学图书馆,杭州,310029 4. 杭州市蔬菜研究所,杭州,316004 |
| 基金项目： | 国家自然科学基金重点资助项目(30230250). |
| 摘 要： | 将具有绿色荧光蛋白(GFP)标记和氯霉素抗性的重组质粒pRP22-GFP导入生防菌Brevibacillus brevis ZJY-1和Bacillus subtilis ZJY-116中，用这些标记菌株处理黄瓜种子，出苗后通过定期分离计数具有上述表型的转化子，研究了2株生防菌在黄瓜根围的定殖规律.结果表明，在整个生育期2株生防菌株均能在黄瓜根围有效定殖，并在黄瓜盛花期和盛果期出现数量高峰.盆栽试验发现，引入黄瓜根围的2株生防菌有向周边杂草迁移的特性，且在前一季寄主植物死亡后，菌株可在下一季植株根围增殖. |
| 关 键 词： | 质粒pRP22-GFP GFP基因 短短芽孢杆菌 枯草芽孢杆菌 黄瓜根围 定殖 |
| 文章编号： | 1001-9332(2005)11-2144-05 |
| 收稿时间： | 2005-03-02 |
| 修稿时间： | 2005-06-13 |
Labeling of biocontrol agents ZJY-1 and ZJY-116 gfp gene and its ecological adaptability in cucumber rhizosphere |
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| ZHANG Xin,ZHANG Bingxin,YU Jingquan,ZHANG Zhen,SHEN Weifeng,CHEN Zhenyu,SHI Jiang.Labeling of biocontrol agents ZJY-1 and ZJY-116 gfp gene and its ecological adaptability in cucumber rhizosphere[J].Chinese Journal of Applied Ecology,2005,16(11):2144-2148. | |
| Authors: | ZHANG Xin ZHANG Bingxin YU Jingquan ZHANG Zhen SHEN Weifeng CHEN Zhenyu SHI Jiang |
| Institution: | 1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China ; 2.Zhejiang Academy of Agricultural Science, Hangzhou 310021, China ; 3.Library of Zhejiang University, Hangzhou 316004, China ; 4.Vegetable Institute of Hangzhou, Hangzhou 316004, China |
| Abstract: | The recombined plasmid pRP22-GFP contained with gfp gene and chloramphenicol resistant was successfully introduced into two biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116.After seed inoculation,the survival and colonization of the two strains were studied by periodically retrieving the GFP-tagged strains in the cucumber rhizosphere based on the selective markers.The results showed that both the strains could successfully colonize in the rhizosphere during the whole life of cucumber,and a higher colonization level was observed during anthesis and fruition stages.In pot trials,they could migrate to the nearby non-inoculated spontaneous weed plants,and reestablish in the rhizosphere of plants subsequently grown in the same pot. |
| Keywords: | Plasmid pRP22-GFP Gfp gene Brevibacillus brevis Bacillus subtili Cucumber rhizosphere Colonization |
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