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重金属污染土壤毒性的发光菌法诊断
引用本文:李彬,李培军,王晶,杨桂芬,张海荣.重金属污染土壤毒性的发光菌法诊断[J].应用生态学报,2001,12(3):443-446.
作者姓名:李彬  李培军  王晶  杨桂芬  张海荣
作者单位:中国科学院沈阳应用生态研究所,
基金项目:国家重点基础研究发展规划项目(G1999011808-5)和中国科学院重大资助项目(KZ951-B1-207-01-03).
摘    要:应用明亮发光杆菌T3(Photobacterium Phosthoreum)对重金属污染土壤的毒性进行诊断,确定了实验室中采用人为定量投加污染物的情况下,土壤的最佳平衡时间为24h,最佳浸提时间为2h,最佳浸提剂为0.1mol

关 键 词:毒性  发光菌法  土壤污染  重金属污染  诊断
文章编号:1001-9332(2001)03-0443-04
修稿时间:2000年7月24日

Photobacterium phosphoreum assay on the toxicity of soil contaminated by heavy metals
LI Bin,LI Peijun,WANG Jing,YANG Guifen,ZHANG Hairong.Photobacterium phosphoreum assay on the toxicity of soil contaminated by heavy metals[J].Chinese Journal of Applied Ecology,2001,12(3):443-446.
Authors:LI Bin  LI Peijun  WANG Jing  YANG Guifen  ZHANG Hairong
Institution:Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110015. libin-libin@hotmail.com
Abstract:The toxicity of soil artificially contaminated by heavy metals was assessed with Photobacterium phosphoreum. The results showed that the duration of optimum balance was 24 hours, and that of optimum extraction was 2 hours. Two leaching methods were compared, and data showed that optimum leaching solvent was hydrocarbon chloride with a concentration of 0.1 mol.L-1 HCL. Dose-response curve indicated that the content of heavy metals was positively related with the toxicity of soil. The general detection limit given by EC50 of soil contaminated by single heavy metal were Cd 26.12 mg.kg-1, Cu 291.48 mg.kg-1, Zn 72.46 mg.kg-1 and Pb 2174.93 mg.kg-1. This study also indicated the toxicity of complex heavy metals pollution in soil was stronger than that of individual element. The research could provide a theoretic base for the remediation of soil contaminated by heavy metals.
Keywords:Soil  Toxicity  Photobacterium phosphoreum    
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