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硅和白粉菌诱导接种对黄瓜幼苗白粉病抗性影响的研究
引用本文:魏国强,朱祝军,李娟,姚秋菊.硅和白粉菌诱导接种对黄瓜幼苗白粉病抗性影响的研究[J].应用生态学报,2004,15(11):2147-2151.
作者姓名:魏国强  朱祝军  李娟  姚秋菊
作者单位:浙江大学园艺系,农业部园艺植物生长发育与生物技术重点开放实验室,杭州 310029
基金项目:国家“8 63”高技术计划资助项目 (2 0 0 1AA2 470 3 1)
摘    要:研究了硅酸盐和诱导接种白粉菌对黄瓜活性氧代谢、SiO2含量和抗病性的影响.结果表明,诱导接种能使叶片的超氧自由基(O2^-)产生速率、H2O2和丙二醛(MDA)含量升高,加硅接种处理的O2^-产生速率、H202和MDA含量明显低于不加硅接种处理.诱导接种能使叶片的过氧化氢酶(CAT)、过氧化物酶(POD)活性升高,超氧化物歧化酶(SOD)活性降低.加硅接种处理植株叶片的CAT、POD和SOD活性明显高于不加硅接种处理.诱导接种提高叶片的抗坏血酸(AsA)和还原型谷胱甘肽(GSH)含量,加硅处理的AsA含量明显低于不加硅处理,GSH含量高于不加硅处理.无论接种与否,加硅处理的SiO2含量显著高于不加硅处理,病情指数明显低于不加硅处理.

关 键 词:黄瓜白粉病    酶活性  抗坏血酸  还原型谷胱甘肽
修稿时间:2003年7月28日

Effects of silicon supply and Sphaerotheca fuliginea inoculation on resistance of cucumber seedlings against powdery mildew
WEI Guoqiang,ZHU Zhujun,LI Juan,YAO Qiuju.Effects of silicon supply and Sphaerotheca fuliginea inoculation on resistance of cucumber seedlings against powdery mildew[J].Chinese Journal of Applied Ecology,2004,15(11):2147-2151.
Authors:WEI Guoqiang  ZHU Zhujun  LI Juan  YAO Qiuju
Institution:State Agriculture Ministry Laboratory of Horticultural Plant Growth Development and Biotechnology, Department of Horticulture, Zhejiang University, Hangzhou 310029, China. veg-phy@zju.edu.cn
Abstract:By the method of hydroponic culture, this paper studied the effects of soluble silicon (Si) supply and Sphaerotheca fuliginea inoculation on the active oxygen metabolism, SiO2 content and disease resistance of a susceptible cultivar. The results showed that the O2*- producing rate and H2O2 and MDA contents of cucumber leaf were increased by Sphaerotheca fuliginea inoculation, but reduced by the inoculation plus Si supply. The inoculation enhanced the catalase (CAT) and peroxidase (POD) activities in plant leaves but decreased their superoxide dismutase (SOD) activity, while inoculation plus Si supply increased the activities of these enzymes. Inoculation increased the scorbate (AsA) and glutathione (GSH) contents in plant leaves. The AsA content in Si-supplied plant leaves was lower than that of Si-deprived ones, whereas the GSH content was in reverse. Whether inoculated or not, the SiO2 content in Si-supplied plant leaves was significantly higher than that of Si-deprived ones. The disease index was significantly decreased by Si-supply.
Keywords:Cucumber powdery mildew  Silicon  Enzyme activity  AsA  GSH  
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