胶州湾浮游植物rbcL基因分子遗传多样性研究

利用聚合酶链式反应扩增胶州湾表层海水浮游植物核酮糖1，5-二磷酸羧化／氧化酶大亚基基因(rbcL)片段，建立了该基因片段变异类型文库．随机测定了28个rbcL片段序列，依此初步分析了胶州湾表层海水浮游植物rbcL基因分子遗传多样性．结果表明，春季胶州湾表层海水浮游植物优势种群为D类rbcL代表的浮游植物，其中隐藻占28．6％、Stramenopies占32．1％、定鞭藻占28．6％、红藻占3．6％．B类rbcL代表的浮游植物为绿藻，占7．1％．根据各操作分类单元丰度计算的分子遗传多样性指数为2．85，根据逆翻译成的氨基酸序列计算的序列多样性为0．20，

Molecular genetic diversity of phytoplankton rbcL gene in Jiaozhou Bay

The variants of a 500 base pair fragment of RubisCo large subunit gene(rbcL)from the phytoplanktonic DNA of Jiaozhou Bay surface seawater were amplified by using polymerase chain reaction and cloned.Twenty-eight clones were randomly selected and sequenced,which were further used to determine the molecular genetic diversity of the phytoplankton of Jiaozhou Bay surface seawater.Systematic analysis showed that the clones representing cryptophyta counted for 28.6%,Stramenopiles 32.1%,Haptophyta 28.6%,Rhodophyta 3.6% and Chlorophyta 7.1%.The sequences from Cryptophyta,Stramenopiles,Haptophyta and Rhodophyta belonged to type D of Form I rbcL and Chlorophyta to type B,indicating that the dominant phytoplankton were those represented by type D rbcL.The genetic diversity index and the reversely translated amino acid sequence diversity of Jiaozhou Bay phytoplankton were 2.85 and 0.20,which were determined by the abundances of operational taxonomy units and the reversely translated amino acid sequences respectively.