红松越冬针叶的电子自旋共振（ESR）波谱

用ESR波谱仪在越冬红松针叶及叶绿体冻干粉中检出了g值分别为2.0044和2.0037的明确的单峰信号。针叶伤害愈重,信号愈强。此信号可被Vc或富含SOD等多种自由基清除剂的沙棘果汁所抑制。针叶受害愈重,沙棘果汁对信号的抑制作用愈强,Vc的抑制作用愈弱。据此认为红松苗越冬伤害和有机自由基的形成有关。在伤害发展的不同阶段,有机自由基的种类组成也不同。

Electron spin resonance spectrum from overwintered korean pine seedlings.

Single-peaked ESR signals with g factor 2.0044 and 2.0037 were clearely shown respecti vely from overwintered korean pine needles and their lyophylized chloroplasts. The more serious the injury of the needles, the stronger the signal. The signals were diminished by adding Vc or fruit juice of Elaeagnus angustifolia containing rich SOD and other free-radical scavengers.The more serious the injury,the more effective of the fruit juice, but Jess of Vc in inhibiting the ESR signals. The results indicate that the winter injury of korean pine seedlings is related to the formation of organic free radicals. The major kinds of the radicals are differ in advancing stages of the injury development.