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青菜幼苗体内几种保护酶的活性对Pb、Cd、Cr胁迫的反应研究
引用本文:任安芝,高玉葆,刘爽.青菜幼苗体内几种保护酶的活性对Pb、Cd、Cr胁迫的反应研究[J].应用生态学报,2002,13(4):510-512.
作者姓名:任安芝  高玉葆  刘爽
作者单位:南开大学生物学系,天津,300071
基金项目:教育部高校骨干教师资助计划项目(A94103),南开大学科技发展基金资助项目.
摘    要:1 引言自从McCord在1969年第一次从牛红血细胞中发现超氧化物歧化酶(SOD)并且证明其功能是清除超氧化物以后,研究者们在植物细胞中也发现了这种酶,并且证明具有同样的功能.后来又发现植物细胞通过多种途径产生活性氧自由基,同时细胞也存在清除这些自由基的多种途径5],两者形成平衡体系.但是,许多逆境因子如寒冷、干旱、干燥、水淹、重金属Al等都能影响植物体内活性氧代谢系统的平衡,产生大量的氧自由基,它们能够启动膜脂过氧化或膜脂脱脂作

关 键 词:青菜  幼苗  保护酶活性  Pb  Cd  Cr  重金属胁迫  毒害
文章编号:1001-9332(2002)04-0510-03
修稿时间:1999年3月11日

Response of some protective enzymes in Brassica chinensis seedlin gs to Pb2+,Cd2+ and Cr6+ stresses
RENAnzhi,GAO Yubao and LIU Shuang.Response of some protective enzymes in Brassica chinensis seedlin gs to Pb2+,Cd2+ and Cr6+ stresses[J].Chinese Journal of Applied Ecology,2002,13(4):510-512.
Authors:RENAnzhi  GAO Yubao and LIU Shuang
Institution:Department of Biology, Nankai University, Tianjin 300071. razn@eyou.com
Abstract:Seedlings of Brassica chinensis were cultured in 1/2 Hoagland solution for seven days before they were treated with Pb2+, Cd2+ and Cr6+. The activities of their superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) responded to the increasing concentrations of the metals, and the responsive characters varied with different metals and different durations of metal treatments. CAT activity decreased with the increase of metal concentrations, and the order of its sensitivity to the three metals was Cd2 +>Cr6+> Pb2+ . SOD activity followed a rise-fall pattern along metal concentration gradient, but its sensitivity decreased with the duration of metal treatment. There was an obvious difference in POD activity to different metals, only when the treatment was proceeded for a period of time.
Keywords:Brassica chinensis  Protective enzymes  Lead  Cadmium  Chromium  
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