| 种植转Bt基因棉土壤中Bt蛋白的分布 |
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| 引用本文： | 付庆灵,陈愫惋,胡红青,李珍,韩晓芳,王朴.种植转Bt基因棉土壤中Bt蛋白的分布[J].应用生态学报,2011,22(6):1493-1498. |
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| 作者姓名： | 付庆灵 陈愫惋 胡红青 李珍 韩晓芳 王朴 |
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| 作者单位： | 1. 华中农业大学资源与环境学院,武汉,430070
2. 华中农业大学资源与环境学院,武汉430070;武汉市园林科学研究所,武汉430081 |
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| 基金项目： | 国家自然科学基金项目(40671087和41001140)资助 |
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| 摘 要： | 采用盆栽试验结合酶联免疫法(ELISA)测定了转Bt基因抗虫棉及常规棉花不同生育期根际及非根际土壤中的Bt蛋白含量.结果表明:红壤、黄棕壤及黄褐土种植转Bt基因棉花后根际土中Bt蛋白含量显著高于非根际土,而种植常规棉花的根际土与非根际土中Bt蛋白含量差异不显著.在初蕾期转Bt基因棉根际土中的Bt蛋白含量为黄褐土＞黄棕壤＞红壤,分别为常规棉根际土的144%、121%和238%;而在盛花期为黄棕壤＞黄褐土＞红壤,分别为常规棉根际土的156%、116%和197%.无论种植转Bt基因棉还是常规棉,供试土壤的根际和非根际土中Bt蛋白含量都随棉花生育期的推进先增加后减少,在盛花期达最大值.整个生育期内,转Bt基因棉根际土中的Bt蛋白含量大于其非根际土;种植转Bt基因棉土壤中Bt蛋白含量高于常规棉,说明转Bt基因棉花的Bt蛋白可释放到根际土中.
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| 关 键 词： | Bt棉花 Bt蛋白 酶联免疫法(ELISA) 根际土 非根际土 |
Distribution of Bt protein in transgenic cotton soils |
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| Fu Qing-Ling,Chen Su-Wan,Hu Hong-Qing,Li Zhen,Han Xiao-Fang,Wang Pu.Distribution of Bt protein in transgenic cotton soils[J].Chinese Journal of Applied Ecology,2011,22(6):1493-1498. |
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| Authors: | Fu Qing-Ling Chen Su-Wan Hu Hong-Qing Li Zhen Han Xiao-Fang Wang Pu |
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| Institution: | FU Qing-ling1,CHEN Su-wan1,HU Hong-qing1,LI Zhen1,HAN Xiao-fang1,WANG Pu1,2(1College of Resource and Environment,Huazhong Agricultural University,Wuhan 430070,China,2Wuhan Botanical Garden Scientific Research Institute,Wuhan 430081,China) |
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| Abstract: | A pot experiment with red soil,yellow brown soil,and yellow cinnamon soil was conducted to detect the Bt protein content in rhizosphere and non-rhizosphere soils at different growth stages of transgenic Bt cotton and common cotton by using enzyme linked immunosorbent assay(ELISA).With the planting of transgenic Bt cotton,the Bt protein content in rhizosphere soil was significantly higher than that in non-rhizosphere soil;while in common cotton soils,there was no significant difference in the Bt protein cont... |
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| Keywords: | Bt cotton Bt protein ELISA rhizosphere soil non-rhizophere soil |
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