两种土壤类型草本植物根系丛枝菌根真菌多样性

将分属12个科的17种宿主植物分别种植于石灰土和紫色土上，120 d后收获取样，通过CTAB法提取共生菌根内AM真菌的DNA，用特异引物U1/U2扩增编码核糖体28S大亚基的rDNA部分序列，并利用聚丙烯酰胺凝胶电泳(PAGE)和银染显影技术显色, 比较了石灰土和紫色土上草本植物根系内丛枝菌根真菌群落的物种多样性.结果表明：生长于石灰土上的17种宿主植物根系内共发现AM真菌条带29种，平均每种宿主植物内8.29种；生长于紫色土上的17种宿主植物根系内发现条带24种，平均每种宿主植物内9.47种.根系内的AM真菌条带包括特异条带和共有条带.经聚类分析发现，AM真菌对宿主植物的侵染有一定的科属专一性,且受土壤环境等外在条件的影响.同时探讨了AM真菌应用于石灰岩地区生态恢复的可能性.

Species diversity of AMF community colonized in herbages roots in calcareous soil and purple soil

A total of 17 herbages belonging to 12 families were selected as the host plants of arbuscular mycorrhizal fungi (AMF). They were grown on calcareous soil and purple soil, respectively, and sampled on the 120th day after seedling emergence. The DNA of AMF in the herbages roots was extracted by CTAB method, the partial rDNA sequence encoding ribosomal 28S big unit was amplified with special primers U1/U2 for fungi, and the PCR products were analyzed with PAGE silver staining method. In calcareous soil, 29 AMF bands were found in the roots of the 17 host plant species, and each host plant was colonized by 829 AMF bands; while in purple soil, only twenty-four AMF bands were found, with 947 bands in each host plant. All the AMF bands included unique bands and common bands. Cluster analysis showed that the AMF colonization in host plant roots was family-specific, and affected by soil factors. The feasibility of applying AMF in ecological restoration of limestone area was also discussed in this paper.