不同生境下刺五加金丝桃甙含量的季节变化

采用高效液相技术对刺五加(Acanthopanax senticosus)金丝桃甙含量的季节动态和生境差异进行了研究.结果表明,刺五加金丝桃甙只存在于刺五加的叶、花和果实中，其中叶和花中的金丝桃甙量较高，果实中含量较低.刺五加叶中金丝桃甙含量随发育节律呈现明显的动态变化.刺五加芽中金丝桃甙含量较低，约为0.013%.随着芽的发育，金丝桃甙含量逐渐增加，5月中旬左右，刺五加幼叶中金丝桃甙的含量可达0087%，此后金丝桃甙含量迅速下降，8月中旬，叶中金丝桃甙的含量仅为0.008%.在不同生境条件下，刺五加叶中金丝桃甙含量的季节变化规律不尽相同.林内生境刺五加叶中金丝桃甙含量较低，在整个生长季节中，只有幼叶中金丝桃甙含量最高，平均为0.15%，而在林隙生境和林缘生境，金丝桃甙含量较高，最高可分别达到0.42%和0.65%.不同生境刺五加花和果实中金丝桃甙含量也有明显的差异.林缘生境金丝桃甙的含量最高，林隙生境次之，林内生境含量最低.叶面积指数和叶片金丝桃甙含量存在明显的负相关关系，叶面积指数越大，金丝桃甙的含量越低.最后，对金丝桃甙含量的季节动态以及金丝桃甙含量与环境因子之间的关系进行了分析.

Seasonal dynamics of hyperin content in Acanthopanax senticosus at different habitats

The study with HPLC technology showed that hyperin only existed in A.senticosus leaf,flower and fruit,and its content was higher in leaf and flower than in fruit.The hyperin content in A.senticosus leaf showed a distinct dynamic change with the rhythm of development.Its content in bud was about 0.013%,which increased gradually with bud development.In mid May,the hyperin content in young leaf reached the maximum (0.087%);while in mid August,it decreased rapidly,with the lowest content about 0.008%.At different habitats,the hyperin content in A.senticosus leaf had different seasonal rhythm,which was low in understory,being at most (0.15%,)and high in forest gap and edge,with the contents of 0.42% and 0.65%,respectively.The same trend was found in A.senticosus flower and fruit.There was a significantly negative correlation between leaf area index (LAI) and hyperin content.