粗毛栓菌和蜡状芽孢杆菌及其共固定菌对pb2+的吸附

将粗毛栓菌菌丝球与蜡状芽孢杆菌共固定为共固定菌.以粗毛栓菌菌丝球、蜡状芽孢杆菌和共固定菌为研究对象,测定不同吸附时间、初始pH、吸附剂浓度和pb2+浓度对3种生物吸附剂吸附pb2+的影响,并将3种生物吸附剂吸附pb2+前后的红外吸收光谱进行分析比较.结果表明:在吸附剂浓度为2g.L-1、pH为5.0、pb2+浓度为50 mg·L-1条件下对pb2+吸附lh效果良好,其吸附率分别为71.7％、91.0％和96.9％.生物吸附剂红外光谱主要由蛋白质、碳水化合物和含硫、磷酸基团的吸收带组成,表明对pb2+吸附起主要作用的官能团是羟基、羧基、磷酸基和含硫基团.

Lead adsorption by Trametes gallica,Bacillus cereus,and their co-immobilized biomaterial

Taking Trametes gallica mycelium pellets,Bacillus cereus,and their co-immobilized biomaterial as bio-adsorbents,this paper studied their Pb2+ adsorption under effects of different contact time,medium initial pH value and Pb2+ concentration,and bio-adsorbent concentration,and compared the infrared spectra of the bio-adsorbents before and after Pb2+ absorption.The Pb2+ adsorption efficiency of the bio-adsorbents was the highest when the bio-adsorbent concentration was 2 g·L-1,initial pH was 5.0,initial Pb2+ concentration was 50 mg·L-1,and contact time was 1 h,with the Pb2+ biosorption rate being 71.7% for the mycelium pellets of T.gallica,91.0% for B.cereus,and 96.9% for the co-immobilized biomaterial.The infrared spectra of the bio-adsorbents were mainly consisted of the absorption zones of protein,carbohydrates,and sulphur-and phosphors-based groups,suggesting that hydroxyl,carboxyl,and sulphur-and phosphate-based groups played important roles in the Pb2+ adsorption by the bio-adsorbents.