| 水稻对低剂量UV-B辐射胁迫的分子应答研究 |
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| 引用本文： | 曾建敏,林文雄,梁康迳,陈芳育,郭玉春,唐莉娜,何华勤,王经源,梁义元.水稻对低剂量UV-B辐射胁迫的分子应答研究[J].应用生态学报,2003,14(6):941-944. |
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| 作者姓名： | 曾建敏 林文雄 梁康迳 陈芳育 郭玉春 唐莉娜 何华勤 王经源 梁义元 |
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| 作者单位： | 福建农林大学农业生态研究所,福州,350002 |
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| 基金项目： | 国家自然科学基金项目 (3 9970 44 1)，教育部优秀青年教师基金(2 0 0 0 11)，福建省自然科学基金资助项目(B0 2 10 0 14 ) |
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| 摘 要： | 采用mRNA差异显示技术，分析了水稻幼苗受低剂量UV-B辐射的mRNA表达丰度差异，获得了18个差异片段，其中15个差异片段在表达水平上有差别，1条为被抑制表达，而UV—B诱导特异表达的为2条．将这2条片段进一步回收、重扩增发现确为特异带型．对其中的一个差异片段RUVB2进行序列测定和国际互联网查询结果表明，该序列位于Oryza sativa第10染色体的OSJNBb0091N21BAC上(登录号：AC091122)，且该cDNA片段与水稻的3个EST序列有95％以上的同源性，而与其它序列同源性较低．
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| 关 键 词： | 分子应答 mRNA差异显示 水稻 UV—B |
| 文章编号： | 1001-9332(2003)06-0941-04 |
| 修稿时间： | 2003年1月28日 |
Molecular responses of rice ( Oryza sativa L. ) to the stress of lowly enhanced UV-B radiation |
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| ZENG Jianmin,LIN Wenxiong,LIANG Kangjing,CHEN Fangyu,GUO Yuchun,TANG Lina,HE Huaqin,WANG Jingyuan,LIANG Yiyuan.Molecular responses of rice ( Oryza sativa L. ) to the stress of lowly enhanced UV-B radiation[J].Chinese Journal of Applied Ecology,2003,14(6):941-944. |
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| Authors: | ZENG Jianmin LIN Wenxiong LIANG Kangjing CHEN Fangyu GUO Yuchun TANG Lina HE Huaqin WANG Jingyuan LIANG Yiyuan |
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| Institution: | Agroecological Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China. |
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| Abstract: | The approach of mRNA differential display (DD) was used to analyze the gene differential expression of rice seedlings subjected to lowly enhanced Ultraviolet B radiation. The results showed that of the eighteen differential fragments, fifteen ones were different in expressional levels, another one was depressed, and the other two, named as RUVB1 and RUVB2 respectively,which could be recovered and re amplified, were specifically induced. Sequence analysis for RUVB2 through Internet BLAST searching indicated that it was more than 95% identities with three rice ESTs, but very low similarity to others in the GenBank, and was mapped on the OSJNBb0091N21BAC(GenBank accession No.:AC091122) in Oryza sativa L. |
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| Keywords: | Molecular responses mRNA differential display Oryza sativa L UV B radiation |
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