|
|||||
|
|
| 转Bt基因水稻根际土壤微生物多样性的磷脂脂肪酸(PLFAs)表征 | |
| 作者姓名： | Liu W Wang ST Chen YX Wu WX Wang J |
| 作者单位： | 1. 河北大学化学与环境科学学院,河北保定,071002 2. 河北农业大学资源与环境科学学院,河北保定,071001 3. 浙江大学环境与资源学院,杭州,310029 4. 石家庄市园林局,石家庄,050021 |
| 基金项目： | 河北省教育厅科学研究计划项目 |
| 摘 要： | 以亲本水稻为对照,应用13C脉冲标记和磷脂脂肪酸技术,分析转Bt基因对水稻根际微生物多样性的影响.结果表明:转Bt基因水稻与亲本水稻根际均以饱和脂肪酸和支链脂肪酸为主,单不饱和脂肪酸次之,多不饱和脂肪酸最少.苗期、拔节期和抽穗期,转成基因水稻根际革兰氏阳性菌(G+)代表性磷脂脂肪酸含量显著低于亲本水稻;革兰氏阴性菌(G-)代表性磷脂脂肪酸含量显著高于亲本水稻.水稻各生育期,转Bt基因未对水稻根际土壤真菌、放线菌磷脂脂肪酸含量造成显著影响,且转Bt基因水稻与亲本水稻根际微生物磷脂脂肪酸13C含量无显著性差异.表明外源Bt基因插入仅对水稻根际微生物多样性造成短暂影响,不具有持续性. |
| 关 键 词： | 转Bt基因水稻 13C标记 磷脂脂肪酸 根际土壤微生物 |
Microbial diversity in rhizosphere soil of transgenic Bt rice based on the characterization of phospholipids fatty acids |
|
| Liu W,Wang ST,Chen YX,Wu WX,Wang J.Microbial diversity in rhizosphere soil of transgenic Bt rice based on the characterization of phospholipids fatty acids[J].Chinese Journal of Applied Ecology,2011,22(3):727-733. | |
| Authors: | Liu Wei Wang Shu-tao Chen Ying-xu Wu Wei-xiang Wang Jing |
| Institution: | LIU Wei1,WANG Shu-tao2,CHEN Ying-xu3,WU Wei-xiang3,WANG Jing4(1College of Chemistry & Environmental Science,University of Hebei,Baoding 071002,Hebei,China,2College of Resources and Environment Science,Agricultural University of Hebei,Baoding 071001,3College of Environmental and Resource Science,Zhejiang University,Hangzhou 310029,4Shijiazhuang City Bureau of Parks and Woods,Shijiazhuang 050021,China) |
| Abstract: | Taking non-transgenic parental rice as the control,and by using 13C pulse-chase labeling method coupled with phospholipid fatty acid(PLFA) analysis,this paper studied the effects of transgenic Bt rice on the microbial diversity in rhizosphere soil.The results showed that in the rhizosphere soils of transgenic Bt rice and its non-transgenic parent,saturated PLFAs and branched PLFAs were the main,followed by monounsaturated PLFAs,and polyunsaturated PLFAs.A significantly lower amount of Gram-positive bacteria... |
| Keywords: | transgenic Bt rice 13C labeling PLFA rhizosphere soil microbial community |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |