磷酸缓冲液对植物脂质过氧化的影响及其与亚硫酸伤害的关系

以亚硫酸模式处理不同类型植物 ,从脂质过氧化方面探讨了磷酸缓冲液的防护作用 .结果表明 ,植物经磷酸缓冲液处理后 ,对亚硫酸伤害具有明显的防护作用 .磷酸缓冲液可稳定细胞膜结构 ,降低MDA含量 ,增强叶绿素———蛋白质的结合度 ,且与低浓度亚硫酸对SOD活性呈协同效应 .磷酸缓冲液的防护作用因植物种类而异 .同一污染环境下 ,MDA含量高 ,叶绿素结合度低者效应显著 .在测试植物中 ,银杏 >连翘 >小麦苗 .

Effect of phosphate buffer on membrane lipid peroxidation of plants and its relation to sulphurous acid harm.

Three kinds of plant treated with sulphurous acid were used to study the protective function of phosphate buffer in respect of membrane lipid peroxidation. The result shows that after the plants were sprayed with phosphate buffer, they had an obvious protective function to the harm caused by sulphurous acid. The phosphate buffer could stabilize the cell membrane structure, decrease the content of MDA, and increase the degree of chlorophyll protein binding. It also had a coordinated effect on SOD activity with sulphurous acid at low concentration. The protective effect of phosphate buffer was different with plant species. In the same polluted environment, the plants with high MDA concentration and low chlorophyll binding degree had greater protective function, and the effect order of tested plants was Ginkgo biloba>Forsythia suspensa>Triticum aestivum.