| 氯苯胁迫对大豆种子萌发的伤害 |
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| 引用本文： | 刘宛,孙铁珩,周启星,李培军,许华夏,杨桂芬,张海荣,齐鹏.氯苯胁迫对大豆种子萌发的伤害[J].应用生态学报,2002,13(2):141-144. |
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| 作者姓名： | 刘宛 孙铁珩 周启星 李培军 许华夏 杨桂芬 张海荣 齐鹏 |
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| 作者单位： | 1. 中国科学院沈阳应用生态研究所陆地生态过程开放研究实验室,沈阳,110015
2. 辽宁大学生物系,沈阳,110036 |
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| 基金项目： | 中国科学院知识创新工程项目 (KZCX2 4 0 1)，国家自然科学基金资助项目 ( 2 98770 28) |
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| 摘 要： | 在实验室人工控制条件下 ,研究了不同浓度 1,2 ,4 三氯苯胁迫对大豆种子萌发和幼苗生长的影响 .结果表明 ,5 0～ 2 0 0 μg·g-1的 1,2 ,4 三氯苯胁迫对大豆种子的发芽率影响甚小 ,但延迟大豆种子的出苗速率 ;30 0 μg·g-1的 1,2 ,3 三氯苯胁迫使种子停止发芽 .在正常条件下 ,大豆种子播种后第 4天其下胚轴超氧化物歧化酶 (SOD)活性达到高峰 ,之后趋于稳定 .不同浓度 1,2 ,4 三氯苯处理使大豆下胚轴SOD活性下降 ,丙二醛 (MDA)、蛋白质含量及下胚轴直径增加 ;幼苗的生长受抑制 ,干重、鲜重有一定程度的下降 ;而鲜重 /干重的比值变化不大 .受害程度随着 1,2 ,4 三氯苯浓度的增加而加重 .初生根比下胚轴对 1,2 ,4 三氯苯胁迫较为敏感 .
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| 关 键 词： | 氯苯胁迫 SOD活性 种子萌发 大豆 伤害 |
| 文章编号： | 1001-9332(2002)02-0141-04 |
| 修稿时间： | 2001年7月19日 |
Chlorobenze-stressing injury of the germination of soybean seed. |
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| LIU Wan ,SUN Tieheng ,ZHOU Qixing ,LI Peijun ,XU Huaxia ,YANG Guifen ,ZHANG Hairong and QI Peng.Chlorobenze-stressing injury of the germination of soybean seed.[J].Chinese Journal of Applied Ecology,2002,13(2):141-144. |
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| Authors: | LIU Wan SUN Tieheng ZHOU Qixing LI Peijun XU Huaxia YANG Guifen ZHANG Hairong and QI Peng |
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| Institution: | Open Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016. liuwan63@hotmail.com |
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| Abstract: | Effects of various doses of 1,2,4-trichlorobenze on seed germination and seedling growth of soybean were studied. The results indicated that 50-200 micrograms.g-1 of 1,2,4-trichlorobenze had no effect on the percentage of seed germination, but delayed the rate of seedling growth. The seed germination was stopped by 300 micrograms.g-1 of 1,2,4-trichlorobenze. The most activity of SOD took place on the 4th day after sowing of soybean seed in the normal condition, then tended to be in a certain level with a little alteration. Under the various dose stress of 1,2,4-trichlorobenze, the activity of SOD in soybean hypocotyl was decreased, while MDA and protein contents as well as diameter of hypocotyls increased. Seedlings growth, fresh weight(FW), dry weight(DW) were inhibited, while the ratio of FW/DW didn't change. Damage-level of wheat seedlings was increased with increasing 1,2,4-trichlorobenze concentrations. Roots were more sensitive to 1,2,4-trichlorobenze-stress than hypocotyls. |
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| Keywords: | trichlorobenze-stress SOD activity Seed germination Soybean Injury |
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