外源褪黑素对干旱胁迫下黑麦草和苜蓿抗氧化能力及养分吸收的影响

以黑麦草和苜蓿为对象,分别叶面喷施和根施100 μmol·L^-1的褪黑素溶液,在干旱胁迫下测定了生物量、丙二醛(MDA)含量、相对电导率、超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性、养分含量(有机碳C、全氮N、全磷P)等指标,研究外源褪黑素对干旱胁迫下植物抗氧化能力及养分吸收的影响。结果表明: 干旱胁迫下,黑麦草和苜蓿的地上、地下生物量显著降低,外施褪黑素能够有效缓解干旱胁迫对黑麦草和苜蓿生长的抑制作用,叶面喷施和根施褪黑素使干旱胁迫下黑麦草的生物量分别增加14.5%和29.6%,苜蓿的生物量分别增加36.6%和49.1%。干旱胁迫下,黑麦草的SOD、POD活性和苜蓿的SOD活性显著降低,外施褪黑素显著提高黑麦草和苜蓿的SOD、POD、CAT活性,减少叶片中MDA的积累,使叶片相对电导率显著下降,抗氧化能力显著提高。干旱和外施褪黑素对黑麦草和苜蓿有机碳含量无显著影响。干旱胁迫下,黑麦草叶片和根中的N、P含量以及苜蓿根中的N含量降低,外施褪黑素提高黑麦草和苜蓿根和叶片中的N、P含量,这表明褪黑素对干旱胁迫下黑麦草和苜蓿的养分吸收有一定的调节作用。施用褪黑素不仅能改善植物的抗氧化能力,还能调节养分吸收以增强植物对干旱胁迫的适应性,而且叶面喷施褪黑素效果好于根施。

Effects of exogenous melatonin on antioxidant capacity and nutrient uptake of Lolium perenne and Medicago sativa under drought stress

To explore the effects of exogenous melatonin on antioxidant capacity and nutrient uptake of plants under drought stress, we used Lolium perenne and Medicago sativa potted seedlings for foliar spraying and root application of 100 μmol·L^-1 melatonin, respectively. We measured the biomass, malondialdehyde (MDA) content, relative conductivity, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities, as well as nutrient contents (organic carbon, total nitrogen, total phosphorus) under drought stress. The results showed that the biomass of L. perenne and M. sativa decreased significantly under drought stress, and that external melatonin application could effectively alleviate the inhibitory effect of drought stress on L. perenne and M. sativa. Foliar spray and root application of melatonin under drought stress enhanced L. perenne biomass by 14.5% and 29.6%, and that of M. sativa by 36.6% and 49.1%, respectively. The SOD and POD activities in L. perenne and SOD activity in M. sativa significantly decreased under drought stress, and exogenous melatonin significantly increased SOD, POD and CAT activities in L. perenne and M. sativa, reduced the accumulation of MDA in leaves, caused a significant decrease in the relative conductivity of leaves, and significantly increased antioxidant capacity. Drought stress and exogenous melatonin did not affect organic carbon content of L. perenne and M. sativa. Under drought stress, the contents of N and P in L. perenne leaves and roots and the content of N in M. sativa roots decreased, while the application of melatonin increased the contents of N and P in roots and leaves of L. perenne and M. sativa, indicating that melatonin could regulate the nutrient absorption of L. perenne and M. sativa under drought stress. In conclusion, the melatonin application not only improved the antioxidant capacity of plants, but also regulated nutrient uptake to enhance plant resilience to drought stress. Foliar spraying of melatonin was more effective than root application.