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NaCl胁迫对菜用大豆种子多胺代谢的影响
作者姓名:Wang C  Zhu YL  Yang LF  Yang HS
作者单位:1. 内蒙古民族大学农学院,内蒙古通辽,028042
2. 国家大豆改良中心,南京农业大学园艺学院,南京210095
基金项目:“十一五”国家高技术研究发展计划重大项目(2006AA10A110); 国家转基因生物新品种培育科技重大专项(2009ZX08004-011B); 内蒙古民族大学科研创新团队计划项目(NMD1003);内蒙古民族大学博士启动基金项目(BS217)资助
摘    要:采用蛭石栽培,在100 mmol·L-1NaCl胁迫下,对耐盐性不同的两个品种菜用大豆种子的丙二醛(MDA)含量和多胺(PAs)代谢进行了研究.结果表明:NaCl胁迫显著增加了菜用大豆种子的MDA含量,但耐盐品种‘绿领特早’(LL)的增幅低于盐敏感品种‘理想高产95-1’(LX).与LX相比,LL种子在整个NaCl胁迫期间均维持了相对较高的游离态精胺(Spm)、结合态Spm、结合态亚精胺(Spd)、束缚态Spd和束缚态腐胺(Put)含量,较高的(Spd +Spm )/Put 和(cPAs+bPAs)/fPAs值及较低的Put/PAs值,在胁迫中、后期(9~15 d)维持了相对较高的游离态Spd含量;胁迫期间,LL的精胺酸脱羧酶(ADC)长时期(6~15 d)保持相对较高的活性,而多胺氧化酶(PAO)则长时期(6~15 d)维持相对较低的活性.综上,LL具有较强的多胺合成能力及较强的Put向Spd和Spm以及游离态多胺向结合态和束缚态多胺转化的能力,进而有效抑制了细胞的膜脂过氧化,这可能是其耐盐性较强的重要原因之一.

关 键 词:NaCl胁迫  菜用大豆种子  游离态多胺  结合态多胺  束缚态多胺

Effects of NaCl stress on polyamines metabolism in vegetable soybean
Wang C,Zhu YL,Yang LF,Yang HS.Effects of NaCl stress on polyamines metabolism in vegetable soybean[J].Chinese Journal of Applied Ecology,2011,22(11):2883-2893.
Authors:Wang Cong  Zhu Yue-Lin  Yang Li-Fei  Yang Heng-Shan
Institution:WANG Cong1,ZHU Yue-lin2,YANG Li-fei2,YANG Heng-shan1(1College of Agriculture,Inner Mongolia University for Nationalities,Tongliao 028042,Inner Mongolia,China,2National Center for Soybean Improvement,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)
Abstract:By adopting vermiculite culture, this paper studied the malondialdehyde (MDA) content and polyamines (PAs) metabolism in the seeds of two vegetable soybean cultivars with different salt tolerance under the stress of 100 mmol NaCl x L(-1). NaCl stress increased the MDA contents in the seeds of the two cultivars significantly, but the increment was lower for salt-resistant cultivar 'Lülingtezao' (LL) than for salt-sensitive cultivar 'Lixianggaochan 95-1' (LX). In the whole period of NaCl stress, LL seeds had higher contents of free spermine (Spm), conjugated Spm, conjugated spermidine (Spd), bound Spd and bound putrescine (Put), higher values of (Spd+Spm)/Put and (cPAs+bPAs)/fPAs, and lower value of Put/PAs, and had higher content of free Spd in the mid and late period (9-15 d) of NaCl stress, as compared with LX seeds. In a longer term (6-15 d) of the stress, LL seeds maintained a higher activity of arginine decarboxylase (ADC) and a lower activity of polyamine oxidase (PAO). All the results suggested that LL seeds had stronger capabilities for polyamines synthesis and for the transformation of Put to Spd and Spm and of free polyamine to conjugated and bound polyamines, which effectively inhibited the membrane lipid peroxidation, possibly being one of the important reasons for the stronger salt tolerance of LL.
Keywords:NaCl stress  vegetable soybean  free polyamine  conjugated polyamine  bound polyamine    
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