| 引进天敌莲草直胸跳甲的遗传多样性 |
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| 引用本文： | 韩政,赵龙龙,马瑞燕,郝炯,贾栋.引进天敌莲草直胸跳甲的遗传多样性[J].应用生态学报,2011,22(11). |
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| 作者姓名： | 韩政 赵龙龙 马瑞燕 郝炯 贾栋 |
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| 作者单位： | 山西农业大学农学院,山西太谷,030801 |
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| 基金项目： | 教育部新世纪优秀人才计划项目(NCET-08-0886); 国家自然科学基金项目(31070480); 山西省归国留学人员科研项目(2009036)资助 |
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| 摘 要： | 我国从美国引进天敌昆虫莲草直胸跳甲防治喜旱莲子草已有20多年历史.本研究采用随机引物扩增多态性DNA(RAPD)分析方法,对引入我国的4个莲草直胸跳甲地理种群(广州、重庆、昆明、福州)的遗传多样性情况进行分析,并以美国佛罗里达种群为参照,从分子水平上揭示其种群的遗传分化及其与环境之间的关系.从111条随机引物中选取13条条带清晰、重复性好的引物对5个种群的25个样本进行扩增.结果表明:总多态位点百分率为42.0％,其中美国佛罗里达种群的多态位点百分率显著高于我国的4个地理种群；25.5％遗传分化发生在4个群体间；对各地理种群间的遗传距离采用非加权配对算术平均法( UPGMA)进行聚类分析发现,遗传距离与种群空间分布距离呈正相关.
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| 关 键 词： | 莲草直胸跳甲 引种 地理种群 遗传多样性 RAPD |
Genetic diversity of introduced natural enemy Agasicles hygrophila |
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| HAN Zheng,ZHAO Long-long,MA Rui-yan,HAO Jiong,JIA Dong.Genetic diversity of introduced natural enemy Agasicles hygrophila[J].Chinese Journal of Applied Ecology,2011,22(11). |
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| Authors: | HAN Zheng ZHAO Long-long MA Rui-yan HAO Jiong JIA Dong |
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| Institution: | HAN Zheng,ZHAO Long-long,MA Rui-yan,HAO Jiong,JIA Dong(College of Agronomy,Shanxi Agricultural University,Taigu 030801,Shanxi,China) |
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| Abstract: | Flea beetle Agasicles hygrophila Selman & Vogt,the most effective natural enemy of alligator weed Alternanthera philoxeroideis,is originated from South America,and has been introduced into China for more than 20 years to control the weed.In this study,the technique of random amplification of polymorphic DNA(RAPD) was applied to assess the genetic diversity of four A.hygrophila geographic populations in Guangzhou,Chongqing,Kunming,and Fuzhou of China,and,taking the population in Florida of US as the referenc... |
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| Keywords: | Agasicles hygrophila introduction geographic population genetic diversity RAPD |
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