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硝酸镧在小鼠肝中的积累及遗传毒理研究
引用本文:王洋,聂刘旺,陈文,张际峰,陈启龙.硝酸镧在小鼠肝中的积累及遗传毒理研究[J].应用生态学报,2005,16(1):133-136.
作者姓名:王洋  聂刘旺  陈文  张际峰  陈启龙
作者单位:安徽师范大学生命科学学院,芜湖,241000
基金项目:国家自然科学基金项目(30370211),安徽省优秀青年基金项目(2002),安徽省教育厅自然科学基金项目(2002kj134),安徽师范大学青年科学基金项目(2002xqn50),重要生物资源保护与利用安徽省重点实验室基金资助项目.
摘    要:通过在饮水中加入硝酸镧使小鼠摄入稀土,1个月后采用ICP-MS法测试了镧在小鼠肝中的积累,研究了硝酸镧对骨髓细胞微核率的影响,并采用体外试验研究了硝酸镧对小鼠基因组DNA的切割作用,探讨了稀土元素镧的遗传毒理.结果表明,1 000、500、300和50 μg·ml-1处理组小鼠肝中镧含量分别达1.46、0.558、0.529和0.083 μg·g-1,与对照组0.028 μg·g-1的含量相比,各处理组小鼠肝中镧的含量皆有升高且与喂饮硝酸镧溶液的浓度成正比(r=0.980).1 000、500和300 μg·ml-1处理组微核率与对照组之间的t检验结果表明各组微核率显著上升(P<0.05),且亦与喂饮硝酸镧溶液的浓度成正比(r=0.853).体外试验显示,硝酸镧可切断DNA链,说明稀土元素镧可在生物体内积累,其在细胞内可使DNA断裂而导致遗传物质受损.

关 键 词:硝酸镧  ICPMS  微核率  DNA断裂  遗传毒理
文章编号:1001-9332(2005)01-0133-04
收稿时间:2004-04-21
修稿时间:2004年4月21日

Accumulation of La(NO3)3 in mice liver and its genetic toxicity
WANG Yang,NIE Liuwang,CHEN Wen,ZHANG Jifeng,CHEN Qilong.Accumulation of La(NO3)3 in mice liver and its genetic toxicity[J].Chinese Journal of Applied Ecology,2005,16(1):133-136.
Authors:WANG Yang  NIE Liuwang  CHEN Wen  ZHANG Jifeng  CHEN Qilong
Institution:College of Life Science, Anhui Normal University, Wuhu, China. yangwang-email@163.com
Abstract:By using ICP-MS method, this paper determined the accumulation of La in mice liver after the mice being fed with La(NO3)3 water solution for thirty days, and through in vitro experiment, studied the effect of La(NO3)3 on the micronucleus rate of mice bone marrow cells and the cleavage action of La(NO3)3 on genome DNA to investigate the genetic toxicity of La. The results showed that when the treated concentration was 1000, 500, 300 and 50 microg x ml(-1), the amount of La in mice liver reached 1.46, 0.558, 0.529 and 0.083 microg x g(-1), respectively. Compared with control, the La amount in disposed groups' mice livers increased with increasing La(NO3)3 concentration in water (r = 0.980). T-test results showed that there existed significant differences in 1000, 500 and 300 microg x ml(-1) disposed groups when compared with the control (P < 0.05). The micronucleus rate of mice bone marrow cells increased with increasing La(NO3)3 concentration in water (r = 0.853). The in vitro experiments showed that La(NO3)3 could make DNA cleaved. It could be concluded that that La might be accumulated in organisms, and could induce the damage of genetic material in cells.
Keywords:La(NO_3)_3  ICP-MS  Micronucleus rate  DNA cleavage  Genetic toxicity  
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