| Bt毒蛋白Cry1Ac在人造土壤中对赤子爱胜蚓毒理及生化影响 |
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| 引用本文： | 肖能文,戈峰,刘向辉.Bt毒蛋白Cry1Ac在人造土壤中对赤子爱胜蚓毒理及生化影响[J].应用生态学报,2005,16(8):1523-1526. |
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| 作者姓名： | 肖能文 戈峰 刘向辉 |
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| 作者单位： | 中国科学院动物研究所,农业虫鼠害综合治理研究国家重点实验室,北京,100080 |
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| 基金项目： | 中国科学院知识创新工程资助项目(KSCX-03-IOZ-4). |
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| 摘 要： | Bt毒素能通过转基因作物的花粉、根和残株进入土壤.为评估转基因作物对土壤动物的影响，本文模拟转基因棉的Bt毒素进入土壤的发生程度，用含不同浓度Bt毒蛋白Cry1Ac的人造土壤处理蚯蚓，测定蚯蚓存活率、重量变化及体内总蛋白含量和过氧化氢酶(CAT)、乙酰胆碱酯酶(AchE)、谷胱甘肽?S?转移酶(GST)和纤维素酶活性.结果表明，Bt毒蛋白对蚯蚓的生物量和生理水平影响均不明显，不存在急毒性和亚致死毒性影响，对蚯蚓比较安全.
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| 关 键 词： | 赤子爱胜蚓 Bt毒蛋白 总蛋白含量 过氧化氢酶 乙酰胆碱酯酶 纤维素酶 谷胱甘肽-S-转移酶 |
| 文章编号： | 1001-9332(2005)08-1523-04 |
| 收稿时间： | 2004-09-02 |
| 修稿时间： | 2004年9月2日 |
Effects of Bt toxin Cry1Ac on biochemical responses of Eisenia fetida in an artificial soil |
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| XIAO Nengwen,GE Feng,Liu Xianghui.Effects of Bt toxin Cry1Ac on biochemical responses of Eisenia fetida in an artificial soil[J].Chinese Journal of Applied Ecology,2005,16(8):1523-1526. |
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| Authors: | XIAO Nengwen GE Feng Liu Xianghui |
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| Institution: | State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China. xiaonw@ioz.ac.cn |
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| Abstract: | Bt toxin Cry1Ac could be introduced into soil by pollen,root exudates,and plant residues.To investigate its toxic effects,the Bt toxin was added into an earthworm-cultured artificial soil,and the survival,growth rate,sperm count,protein content,and activities of acetylcholinesterase (AchE),catalase (CAT),glutathione-S-transferase (GST) and cellulase in Eisenia fetida were determined.The results suggested that Bt toxin Cry1Ac had little effects on the biomass and physiological characteristics of E.fetida,with no acute and subchronic toxicity,and was safe for earthworm at field dose level. |
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| Keywords: | Eisenia fetida Bt toxin Cry1Ac Protein content CAT AchE Cellulase GST |
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