氯化镉对葡萄根系线粒体特性与根系活力的影响

以巨峰、玫瑰香、龙眼和泽香4个葡萄品种一年生扦插苗为试材，研究了氯化镉（CdCl₂）处理后葡萄根系线粒体过氧化氢（H₂O₂）、膜通透性转换孔（MPTP）、膜电位（Δψ）、细胞色素C（Cyt c）及根系活力的变化.结果表明：4个葡萄品种经0.5 mmol·L^-1的CdCl₂处理后，根系线粒体H₂O₂含量上升，MPTP开放程度增大，Δψ降低，Cyt c含量下降，根系活力明显降低;其中，H₂O₂含量和Cyt c 含量由高到低的顺序为：巨峰＞泽香＞玫瑰香＞龙眼，而根系活力、MPTP和Δψ由高到低的顺序为：龙眼＞玫瑰香＞泽香＞巨峰.表明巨峰葡萄根系活力比其他品种更易受到CdCl₂的影响，而龙眼葡萄根系活力在4个品种中受CdCl₂影响最小.

Effects of CdCl2 on grape root mitochondrial characteristics and root activity.

Taking the one-year cutting-seedlings of four grape cultivars (Kyoho, Muscat Hamburg, Long Yan, and Ze Xiang) as test materials, their root mitochondr ial hydrogen peroxide (H2O2) content, membrane permeability transition pore (MPTP), membrane potential (Δψ), cytochrome C (Cyt c) content, and root activity were measured under effects of CdCl2. For all test grape cultivars, the root mitochondrial H2O2 content and MPTP increased, but the Δψ, Cyt c content, and root activity decreased after treated with 05 mmol CdCl2·L-1. The m itochondrial H2O2 content and Cyt c content were in the sequence of Kyoho>Ze Xiang>Muscat Hamburg>Long Yan, while the root activity, MPTP, and Δψ were in ad verse, i.e., Long Yan>Muscat Hamburg>Ze Xiang>Kyoho. Among the test grape cultiv ars, the root activity of Kyoho was easier to be inhibited under Cd stress, while t hat of Long Yan was least affected by CdCl2.