水分胁迫对不同基因型小麦幼芽蛋白质表达和某些生理特性的影响

以抗旱小麦品种长武134和不抗旱小麦品种郑引1号为试材，采用-1.2 MPa PEG 6000处理种子，研究不同水分条件下小麦幼芽中蛋白质表达和部分生理特性的变化.聚丙烯酰胺凝胶电泳结果表明：水分胁迫可诱导抗旱品种幼芽产生分子量约39.5 kDa和23.0 kDa两种新蛋白亚基，不抗旱品种则无新亚基产生；在正常供水条件下，随着生育期延长，两品种中分子量为48.5 kDa的蛋白亚基表达量逐渐增强，因其对水分敏感且属于新发现蛋白，初步命名为水敏感蛋白.生理特性测定结果表明，水分胁迫条件下长武134的根芽比和相对含水量均高于郑引1号，而细胞膜相对透性和丙二醛含量则低于郑引1号.

Effects of water stress on protein expression and physiological properties of different genotype wheat(Triticum aestivum L.)sprouts

With drought-resistant wheat(Triticum aestivum)cuhivar Changwu 134 and droughtsensitive cuhivar Zhengyin 1 as test materials,and by using-1.2 MPa PEG 6000 to treat their seeds,this paper studied the protein expression and physiological properties of the sprouts under different soil moisture conditions.SDS-PAGE analysis showed that water stress induced the production of two new proteins with molecular weights of 39.5 kDa and 23.0 kDa in Changwu 134 but not in Zhengyin 1 sprouts.Under normal water supply,the expression of the protein with molecular weight of 48.5 kDa in the sprouts of both Changwu 134 and Zhengyin 1 increased with sprout growth.This protein was preliminarily named as water-sensitive protein,due to lts wate-sensitivity and of newly discovered protein.The determinations of physiological properties showed that under water stress,the sprouts of drought-resistant Changwu 134 had higher root/shoot ratio and higher relative water content,but lower relative membrane permeability and lower malondlaldehyde content than those of drought-sensitive Zhengyin 1.