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水稻化感材料控制稗草的基因定位研究
引用本文:徐正浩,何勇,崔绍荣,赵明,张旭,李迪.水稻化感材料控制稗草的基因定位研究[J].应用生态学报,2003,14(12):2258-2260.
作者姓名:徐正浩  何勇  崔绍荣  赵明  张旭  李迪
作者单位:1. 浙江大学生物系统工程及食品科学学院,杭州,310029;中国水稻研究所,杭州310006;中国农业大学作物学院,北京,100094
2. 浙江大学生物系统工程及食品科学学院,杭州,310029
3. 中国农业大学作物学院,北京,100094
4. 中国水稻研究所,杭州310006
基金项目:国家自然科学基金项目 (3 0 170 62 0 ),国家“十五”科技攻关资助项目 (2 0 0 0 1BA5 0 9B0 7)
摘    要:利用中156/谷梅2号建立的重组自交系(RILs)所构建的包括168个DNA标记,全长为1447.9cM。基本覆盖水稻基因组12条染色体的连锁图,用差时播种共培法的改进方法对134个该群体的株系及其亲本对无芒稗进行了化感作用评价,用无芒稗的植株干重作为表型定位水稻化感控制稗草的基因,用QTL Mapper 1.01b软件进行区间作图,检测到1个与化感作用有关的主效应QTL。该QTL位于第7条染色体上,解释了32.30%的表型变化;检测到6对上位QTL,解释了47.83%的无芒稗干重抑制的变化,主效应和上位效应QTL共解释了80.13%的表型变化。

关 键 词:水稻  差时播种共培法  化感作用  无芒稗  QTL
文章编号:1001-9332(2003)12-2258-03
修稿时间:2003年7月19日

Genes mapping on rice allelopathy against barnyardgrass
XU Zhenghao ,,HE Yong ,CUI Shaorong ,ZHAO Ming ,ZHANG Xu ,LI Di.Genes mapping on rice allelopathy against barnyardgrass[J].Chinese Journal of Applied Ecology,2003,14(12):2258-2260.
Authors:XU Zhenghao      HE Yong  CUI Shaorong  ZHAO Ming  ZHANG Xu  LI Di
Institution:College of Biosystem Engineering and Food Science, Zhejiang University, China National Rice Research Institute, Hangzhou, China. xzh640909@sohu.com
Abstract:By using the relay seeding technique with slight modification, a population of 134 recombinant inbred lines (RILs) derived from a cross between indica rice (Oryza sativa L.) cultivar Zhong 156 x Gumei 2 and contained 168 DNA markers covering all 12 chromosomes with 1447.9 cM spans was employed to evaluate the rice allelopathic effect on barnyardgrass Echinochloa crus-galli (I.) Beauv.]. The phenotyping values of the biomass of barnyardgrass infested with rice materials were employed to map the genes of allelopathic activity on barnyardgrass. One main effect of QTL on 7 chromosomes was identified, which explained 32.30% of the phenotypic variation. Six pairs of digenic epistatic loci were also detected, which collectively explained 47.83% of the phenotypic variation. The main QTL and epistatic loci totally explained 80.13% of the phenotypic variation of allelopathic activity.
Keywords:Rice  Relay seeding technique  Allelopathic effect  Barnyardgrass  Quantitative trait loci (QTL)
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