微卫星多态性检测技术及其在保护遗传学中的应用

微卫星是一种新型的分子遗传标记，具有只需极少量样品即可进行PCR扩增等优点，使之极其适合于保护遗传化学研究。阐释了微卫星的结构、微卫星多态检测技术的原理，简要论述了微卫生多态检测技术的优点及其应用，并介绍了一种分离动物微卫星位点的富集方法。

Detection technique of microsatellites polymorphism and its application in conservation genetics

Microsatellites are a novel molecular genetic marker,and offer unusual advantages,e.g., with the using of a very few sample, they can be amplified by PCR,which are particularly appropriate in the research of conservation genetics. In this paper,the structure of microsatellites and the principle of the detection technique of microsatellites polymorphism were illustrated, and the advantages and the application of this detection technique were described briefly. For the isolation of microsatellite loci in animals,an enrichment method was introduced. And then,some practical problems of this technique were summarized.