培养条件对海洋假单胞菌脂肪酸的影响

研究了不同温度条件下一株海绵附生假单胞菌（Pseudomonas sp.）在不同碳源培养基中的生长情况及脂肪酸变化.结果表明，该海洋菌生长最适温度为30 ℃，在以淀粉作为外加碳源的培养基中生长最好;实验菌含13种脂肪酸，主要是c16:1(n₇)、c15:0、c16:0、c17:0、c18:1(n₆)、c18:1(n₉)、9,10 cp c17:0和其同分异构体.在30 ℃温度条件下，不饱和脂肪酸的相对含量急剧减少.在有外加碳源(葡萄糖和淀粉)的培养基中生长的细菌，奇数脂肪酸和环丙基脂肪酸含量远比未外加碳源的低.聚类分析结果表明，两种环境因子中，温度比碳源的影响更明显.

Effects of culture condition on fatty acids in Pseudomonas sp

The investigation on the growth of Pseudomonas sp. and the dynamics of its fatty acids under various culture conditions indicated that the optimum temperature for its growth was 30 degrees C, and the best carbon source was starch. 13 kinds of fatty acids were detected, among which, c16:1(n-7), c15:0, c16:0, c17:0, c18:1(n-6), c18:1 (n-9), 9, 10-cp-c17:0 and iso9, 10-cp-c17:0 were the major ones. When cultured at 30 degrees C, the relative content of unsaturated fatty acids decreased significantly. Carbon source in the media was the crucial factor affecting the contents of odd- and cyclopropane fatty acids. Cluster analysis revealed that temperature had a more obvious effect than carbon source.