蜡状芽孢杆菌菌株Jp-A的分离鉴定及其降解苯酚特性

从某钢铁厂处理废水的活性污泥中驯化分离一株能高效降解苯酚的细菌（Jp-A）.通过形态观察、生理生化实验和16srRNA序列分析，初步鉴定Jp A为蜡状芽孢杆菌（Bacillus cereus）.在实验条件下，该菌在16、24和32 h内能将浓度分别为5、10和15 mmol·L^-1的苯酚完全降解，而30 mmol·L^-1的苯酚则完全抑制该菌的生长.该菌也能以甲苯、氯酚类和硝基酚类等芳香烃类物质作为唯一碳源和能源生长.双加氧酶检测表明，其通过间位途径开环裂解苯酚，该途径的关键酶邻苯二酚2，3 双加氧酶主要定位在细胞膜上，为诱导酶，补加葡萄糖能抑制该酶的产生.

Isolation and identification of Bacillus cereus strain Jp-A and its capability in phenol degradation

In this study, a bacterial strain named as Jp-A was isolated from the activated sludge of a steel factory. Through morphological observation,physiological and biochemical test, and 16srRNA sequence analysis, the strain was identified as Bacillus cereus. Under laboratory condition, the phenol at its initial concentration of 5,10 and 15 mmol x L(-1) could be completely degraded by the strain within 16,24 and 32 h, respectively, but the growth of the strain was inhibited when the phenol concentration was 30 mmol x L(-1). The strain could also utilize and degrade other aromatic hydrocarbons, e. g. , benzene, toluene, chlorophenol and nitrophenol, as its sole source of carbon and energy. Dioxygenase test indicated that the strain degraded phenol via meta-pathways. The key enzyme catechol 2,3-dioxygenase was an induced enzyme, which was mainly located on the cell membrane, and adding glucose could inhibit its generation.