我国地带性土壤中产蛋白酶细菌生态分布研究

按我国土壤地理地带分布,从北到南采土样32个,从中分得细菌491株,并对每一菌株进行了形态学观察与描述。用透明圈法半定量测定各菌株产蛋白酶的活力。对其中产酶活性较高的菌株进行了摇瓶液体培养,定量测定了产蛋白酶的活力。根据细菌分布的数量与活性,分析了生态条件与产酶细菌分布的规律,得出了一些有意义的结果。

Study on ecological distribution of protease-producing bacteria in zonal soils

From north China to south China, 32 samples of various types of soil were collected and main ecological factors which might affect the distribution of protease-producing bacteria were studied. Through the study, 491 strains of bacteria were obtained and purified. All of them were systematically observed and their protease-producing activity were half-quantitatively examined. The probable high yielding strains were chosen out and cultured by shaking-flask liquid fermentation method and their protease activity was quantitatively measured. Based on the different protease activity and distribution of bacteria, the relationship between ecological condition and distribution of protease-producing bacteria was studied and following conclusion was obtained: the ecological distribution of protease-producing bacteria has some positive relation with the amounts of soil organic matter and nitrogen.