褐飞虱高温条件下应激反应及体内保护酶系活性的研究

利用34℃、36℃、38℃3个温度处理褐飞虱的若虫和不同型成虫，研究其在热休克过程中体内保护酶系活性及脂质过氧化物含量的变化。结果表明，热休克对4龄若虫过氧化氢酶（CAT）活性影响大于3龄、5龄，而其成虫期CAT活性则随日龄增加而增加，36℃为CAT清除H2O2最适温度。高龄若虫全内谷胱甘肽过氧化物本(GSH-px）活性高于低龄若虫，老熟成虫体内GSH－px清除H2O2能力强于初羽化成虫，超氧化物歧化酶（SOD）活性与处理温度呈正相关，而同一处理温度下SOD活性随成虫龄期和成虫日龄增加而降低，长型成虫体内CAT、GSH－px、SOD活性均高于短翅型，而雌、雄成虫间CAT、GSH－px、SOD活性无明显差异（α＞0.05）。褐飞虱体内脂质过氧化物（LPO）含量随处理温度升高而上升，同一处理温度下随若虫龄期和成虫日龄的增加而增加，短型成虫体内LPO含量高于长翅型。

Stress response of Nilaparvata lugens at high temperature and activities of its protective enzyme systems

The nymphae and different wing-form adults of Brown Planthopper (BPH) were treated intermittently at 34 degrees C, 36 degrees C and 38 degrees C. During the process of heat shock treatment, the variation of the activities of the endogenous enzymes of protective system and the content of lipid peroxides (LPO) in BPH were researched. The results showed that the effect of heat shock treatments on CAT activity in 4th instar of nymphae was higher than that in 3rd and 5th instar. The CAT activity in BPH adults increased with the day-age, and 36 degrees C was the suitable temperature at which H2O2 was scavenged by CAT. The GSH-px activity was higher in old than that in young nymphae, and the ability of GSH-px to scavenge H2O2 was higher in old adults than in those adults emerged early. The activity of SOD had a positive correlation with the treated temperatures, and decreased with the increase of nymphae instar and the day-age of adults after emergence at the same temperature. The activities of CAT, GSH-px, and SOD in macropterous adults were higher than those in brachypterous adults. There were no significant difference for the activities of CAT, GSH-px and SOD between female and male adults (alpha > 0.05). The content of LPO in BPH increased with the rise of treated temperature, and also increased with the increase of instars in nymphae and day-age after emergence in adults at the same temperature. The LPO content was higher in brachypterous than in macropterous adults.