不同区域松蓝根、叶(板蓝根、大青叶)有效成分含量差异

以靛蓝、靛玉红为指标性成分，采用HPLC法对不同生长环境(产地)的板蓝根和大青叶中有效成分进行测定.结果表明，不同产地的板蓝根、大青叶中有效成分含量存在显著差异.板蓝根的有效成分总含量以陕西汉阴为最高(16.27 mg·kg^-1)，其次为宁夏隆德(15.67 mg·kg^-1)、安徽亳州（14.90 mg·kg^-1)和安徽临泉(14.23 mg·kg^-1),黑龙江佳木斯最低(13.97 mg·kg^-1 )；大青叶中有效成分总含量以陕西汉阴为最高(698.32 mg·kg^-1)，其次为宁夏隆德(683.68 mg·kg^-1)、安徽亳州(680.11 mg·kg^-1)和安徽临泉(654.19 mg·kg^-1),黑龙江佳木斯最低(642.73 mg·kg^-1)；各产地大青叶HPLC图谱有所差异，不仅表现在指标性成分的峰面积所占比例不同，其成分的种类也存在较大差异.结合产品外观质量和内在品质等因素可以认为，菘蓝地道产区仍需进一步调查分析和确定.

Effective constituents contents in indigowoad roots and leaves from different re-gions

By the method of HPLC, and with indigo and indirubin as indicative constituents, this paper determined the effective constituents contents in indigowoad roots and leaves collected from different regions. The results showed that the total content of effective constituents in indigowoad root was the highest (16.27 mg x kg(-1)) from Hanyin of Shaanxi Province, followed by Longde of Ningxia Autonomous Region (15.67 mg x kg(-1)), Bozhou of Anhui Province (14.90 mg x kg(-1)), and Linquan of Anhui Province and Jiamusi of Heilongjiang Province (13.97 mg x kg(-1)), and that in indigowoad leaf had the same order, with the values being 698.32, 683.68, 680.11, 654.19 and 642.23 mg x kg(-1), respectively. The HPLC chromatograms of leaf samples collected from different regions were differed, not only in the test constituents, but also in the other constituents manifested as various peaks in the chromatogram. It was suggested that besides meteorological factors, the selection of geo-authentic region should be also involved in the cultivation of Isatis indigotica.