福建省烟粉虱田间种群抗药性发展及其影响因素

采用成虫浸叶生测法对福建省不同地区烟粉虱田间种群的抗药性发展进行监测.结果表明:福建各地烟粉虱田间种群对氯氟氰菊酯、甲氰菊酯、毒死蜱仍保持较高水平抗性,对灭多威的抗性水平较低,对阿维菌素未产生明显的抗药性;烟粉虱田间种群对烟碱类杀虫剂的抗性发展极为迅速,其中漳州种群对吡虫啉、噻虫嗪已由2005年的中等水平抗性(抗性倍数分别为23和25倍)发展为2009年的高水平抗性(抗性倍数分别为103和228倍),其他地区种群对吡虫啉、噻虫嗪也由2005年的低水平抗性(抗性倍数分别为1.5～3.3倍和1.7～5.5倍)发展为2009年的中等水平抗性(抗性倍数分别为23 ～ 33倍和29～49倍).采用mtDNA CO Ⅰ分子标记技术对福建省不同地区和寄主植物上采集的8个烟粉虱种群的生物型进行鉴定发现,采自漳州变叶木上的烟粉虱种群为本地土著种(未知生物型),其他7个采自大田蔬菜作物的烟粉虱种群均为B型.寄主植物和温度对烟粉虱种群的药剂敏感性影响有限,吡虫啉等烟碱类杀虫剂高强度使用是B型烟粉虱田间种群对该类型药剂迅速形成抗性的重要原因.

Development of insecticide resistance and its effect factors in field population of Bemisia tabaci in Fujian Province, East China

Abstract: The development of insecticide resistance in field populations of Bemisia tabaci from different regions of Fujian Province, China were monitored with adult leaf-dipping bioassay. Compared with bioassay data of 2005, all field populations tested in 2009 still had high levels of resistance to the lambda-cyhalothrin, fenpropathrin and chlorpyrifos, low levels of resistance to methomyl, and no resistance to abamectin. However, resistance to the neonicotinoids imidacloprid and thiamethoxam had increased from moderate levels (23 folds and 25 folds, respectively) in 2005 to high levels (103 folds and 228 folds, respectively) in 2009 in Zhangzhou population, and low levels (1.5-3.3 folds and 1.7-5.5 folds, respectively) in 2005 to moderate levels (23-33 folds and 29-49 folds, respectively) in 2009 for other populations. The biotype of eight B. tabaci populations collected from different regions and hosts were determined according to the mitochondria cytochrome oxidase I (mtCO I ) sequence, and two different biotypes (B-biotype and indigenous whitefly) were detected. The samples collected from geographical regions and hosts showed a prevalence of the B-biotype, and indigenous whitefly (unknown biotype) existed only in croton of Zhangzhou. Host plants and temperature had limited effects on insecticide development in B. tabaci, while extensive and high frequent use of neonicotinoids could be the most important factors responsible for rapid development of insecticide resistance in field populations of B-biotype B. tabaci.