| 60Coγ射线与EMS复合处理对山黧豆抗氧化酶活力及ODAP含量的影响 |
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| 引用本文： | 覃新程,王飞,王晓娟,周功克,李志孝.60Coγ射线与EMS复合处理对山黧豆抗氧化酶活力及ODAP含量的影响[J].应用生态学报,2000,11(6). |
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| 作者姓名： | 覃新程 王飞 王晓娟 周功克 李志孝 |
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| 作者单位： | 兰州大学干旱农业生态国家重点实验室和应用有机化学国家重点实验室，兰州 730000 |
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| 基金项目： | 国家自然科学基金资助项目!(39770 4 69) |
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| 摘 要： | 1 引 言山黧豆抗寒、抗旱、耐贫瘠 ,尤其适于干旱、半干旱地区种植 .但山黧豆中含有的毒素 β ＯＤＡＰ会导致下肢瘫痪 .由于这个原因限制了山黧豆的大面积栽培 .我们采用 60 Ｃｏγ射线和ＥＭＳ单因子、复因子诱变的方法选育丰产、低毒、无毒山黧豆新品系 .近年来对生物辐射敏感性的研究表明 ,其抗性除与本身ＤＮＡ损伤修复能力有关外 ,还与体内所含防护物质有关 ,ＳＯＤ、ＰＯＤ、ＣＡＴ即是重要的保护物质 .关于抗氧化酶活性与辐射剂量、诱变剂浓度关系的研究很少 ,因此本实验对山黧豆成苗率、抗氧化酶活性、和 β ＯＤＡＰ含量进行…
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| 关 键 词： | ODAP(3-N-Oxalyl-α β-diamino propionic acid) |
Effect of combined treatment of 60Coy-ray and EMS on antioxidase activity and ODAP content in Lathyrus sativus. |
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| QIN Xincheng,WANG Fei,WANG Xiaojuan,ZHOU Gongke,Li Zhixiao.Effect of combined treatment of 60Coy-ray and EMS on antioxidase activity and ODAP content in Lathyrus sativus.[J].Chinese Journal of Applied Ecology,2000,11(6). |
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| Authors: | QIN Xincheng WANG Fei WANG Xiaojuan ZHOU Gongke Li Zhixiao |
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| Abstract: | Lathyrus sativus seeds were treated with 60Coγ-ray and EMS(ethyl methane sulfonate), and their emergence rate and SOD,POD and CAT activities were determined. The result indicated that the treatment decreased the emergence rate. The activities of SOD and POD were changed in accordance with the increase of irradiation dose and EMS concentration, while that of CAT had no obvious change. After treatment, the ODAP content in Lathyrus sativus decreased. Amutant was developed, with toxin content of 0.1%, compared to 0.2% in control. |
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| Keywords: | Lathyrus sativus EMS 60Coy-ray(unit Krad) |
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