转Bt基因抗虫棉叶面可培养微生物多样性的变化

在大田栽培条件下，以转Bt基因抗虫棉GK-12和常规棉泗棉3号为材料，在棉花的苗期、现蕾期、花铃期和吐絮期分别测定棉花叶面细菌、真菌和放线菌的数量变化，并在花铃期和吐絮期对叶面细菌生理群的数量和多样性进行分析.结果表明：棉花叶面可培养微生物数量与其生长发育呈正相关，叶面可培养微生物的数量一般由苗期开始增多，到花铃期达最高峰，吐絮期明显减少；花铃期转Bt基因抗虫棉叶面细菌生理群Simpson指数、Shannon-Wiener指数和均匀度比常规棉升高，吐絮期比常规棉下降.

Changes in diversity of culturable microorganisms on leaf surface of transgenic Bt cotton

In a field study,the quantitative changes of bacterial,actinomycetic and fungal populations on the leaf surface of transgenic Bt cotton(GK-12)and non-transgenic Bt cotton(Simian No.3)were monitored at seedling,squaring,flowering and boll-opening stages,respectively,and the diversity of bacterial physiological groups was analyzed at flowering and boll-opening stages.The results showed that there was a positive correlation between the quantity of culturable microorganisms and the development of cotton.The total number of bacteria,fungi and actinomyces began to rise at seedling stage,reached its peak at flowering stage,and decreased markedly at boll-opening stage.The Simpson index,Shannon-Wiener index,and evenness index of bacterial physiological groups were higher at flowering stage,but lower at boll-opening stage on GK-12 than on Simian No.3.