壬基酚对鲫鱼原代肝细胞增殖和抗氧化功能的影响

研究了不同浓度壬基酚对鲫鱼肝细胞增殖和抗氧化系统的影响.结果表明：各试验浓度壬基酚均能抑制鲫鱼肝细胞的增殖,其中高浓度的壬基酚（10^-3 mol·L^-1）对细胞增殖的抑制作用极其显著，肝细胞形态发生明显改变;壬基酚破坏了鲫鱼肝细胞抗氧化系统的平衡,经壬基酚处理后的肝细胞超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性均受到抑制,而羟自由基的含量升高;壬基酚对原代鲫鱼肝细胞造成氧化损伤,引起培养液中丙二醛（MDA）含量升高.壬基酚诱导的氧化胁迫对原代鲫鱼肝细胞产生了一系列的体外毒性效应.

Effects of nonylphenol on proliferation and anti-oxidative functions of Carassius auratus primary culture hepatecytes.

The study on the proliferation and anti-oxidative functions of Cara ssius auratus primary culture hepatecytes under effects of different concentra tion nonylphenol showed that at all test concentrations of nonylphenol, the prol iferation of the hepatecytes was inhibited. High concentration (10-3 mol· L-1) nonylphenol had significant inhibitory effect, and induced an apparen t morphological alteration of the hepatecytes. Nonylphenol upset the balance of hepatecytes anti-oxidative system through decreasing the activities of superoxi de dismutase (SOD) and catalase (CAT) while increasing the hydroxyl free radical concentration. Nonylphenol also caused the oxidative damage on the hepatecytes, resulting in the increase of malondialdehyde (MDA) concentration in cell cultur e medium. The nonylphenol-induced oxidative stress engendered a series of in vi tro toxic effects on the primary culture hepatecytes of C. auratus.