Phenotypic analysis of boldit Galr1 knockout mice reveals a role for GALR1 galanin receptor in modulating seizure activity but not nerve regeneration |
| |
Authors: | Arie S Jacoby Fiona E Holmes Yvonne J Hort John Shine and Tiina P Iismaa |
| |
Institution: | (1) The Garvan Institute of Medical Research, St. Vincent's Hospital, Sydney, Australia;(2) University Research Centre for Neuroendocrinology, University of Bristol, Bristol, United Kingdom |
| |
Abstract: | The GALR1 galanin receptor is expressed at high levels withinthe central nervous system and is hypothesised to play asignificant role in many of the central actions of galanin. Todetermine which specific actions of galanin are mediated byGALR1, we have developed mice that carry an insertionalinactivating mutation within the first coding exon of the geneencoding GALR1 (Galr1). HomozygousGalr1
-/-mice are viable. Both male and female mice exhibit reducedcirculating levels of insulin-like growth factor-I (IGF-I) butno significant difference in growth rate relative to Galr1
+/+ controls. Female homozygousGalr1
-/-mice are capable of breeding and nursing offspring. Functionalrecovery after sciatic nerve crush is not significantlydifferent in Galr1
-/- mice relative to Galr1
+/+ controls, indicating that GALR1 does not mediate the nerve regenerative effects of galanin. However, homozygous Galr1
-/- mice exhibit spontaneous seizures, identifying a critical role for GALR1 in mediating the anti-seizure activity of galanin. |
| |
Keywords: | epilepsy IGF-I knockout mouse mammary gland neuropeptide sciatic nerve seizures |
本文献已被 SpringerLink 等数据库收录! |