Preparation of Chloroplast DNA from Barley and Lettuce and Comparison of Restriction Fragments |
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Authors: | Mercedes Martin R Tomás María Cruz Izquierdo C Prádanos B Sabater |
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Institution: | (1) Dpto. de Biologia Vegetal, Universidad de Alcala de Henares, Apdo. 20, Alcalá de Henares, Madrid, Spain |
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Abstract: | Chloroplast DNA from three barley cultivars and from one lettuce cultivar was prepared from chloroplasts isolated by Conventional
differential centrifugation. Barley chloroplast DNA size was sensibly lower (130 kpb) than lettuce chloroplast DNA (150 kpb).
Chloroplast DNAs from the three barley cultivars showed similar restriction fragment patterns after digestion with: BamHI,
EcoRI or HindIII. The lettuce chloroplast DNA restriction pattern was very different from the barley chloroplast DNA restriction
pattern. |
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Keywords: | Hordeum vulgare L (cvs Albacete 6-Carreras Hassan) Lactuca saliva L (cv Romana) |
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