Development and evaluation of expressed sequence tag-derived microsatellite markers for hop genotyping |
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Authors: | J Patzak and J Matou?ek |
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Institution: | (1) United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Clonal Germplasm Repository (NCGR), 33447 Peoria Road, Corvallis, OR 97333, USA;(2) USDA, ARS, National Forage Seed Research Center (NFSPRC), Corvallis, OR 97331, USA |
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Abstract: | The use of expressed sequence tag-simple sequence repeat (EST-SSR) markers might reflect the better relationship among species
or cultivars than markers previously used. The first set of 30 EST-SSR was developed in hop (Humulus lupulus L.). They represent 25 gene loci with total of 1268 EST sequences. They were used for characterization of 11 hop samples
and cross-amplification in Humulus japonicus Sieb. et Zucc. The number of alleles per locus ranged from two to nine. The observed and expected heterozygosities ranged from 0.182
to 0.956 and from 0.233 to 0.775, respectively. We used EST-SSR markers for cluster analysis of hop genotypes. Dendrogram
well matched with genealogical and geographical data for hop genotypes. |
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Keywords: | |
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