Pepper seed germination assessed by combined X-radiography and computer-aided imaging analysis |
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Authors: | A Dell’Aquila |
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Institution: | (1) Institute of Plant Genetics, CNR, Via Amendola 165/a, I-70126 Bari, Italy |
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Abstract: | A lot of pepper seeds having 87 % germination were subjected to X-ray inspection using a non lethal dose of radiation. Seeds
with less than 2.7 % (on the basis of total seed area) of free space area, i.e. the spaces between embryo and endosperm, were classified as highly viable seeds (97–100 % germination) with the lowest level
of abnormal seedlings. Seeds X-ray classified as good were subjected to a computerised image analysis to study seed imbibition
and radicle elongation. The patterns of seed area increase, chosen as the most accurate indicator of seed swelling, resembled
the triphasic curve of water uptake. The first phase was completed at 9 h followed by a second phase that varied widely in
time until completion of germination between 52 and 96 h. The proportion of seeds with radicle protrusion between 52–56 h
and 64–72 h assessed with the image analysis was significantly higher than that recorded using a conventional germination
test. In addition, the rate of increase of seed area during the third phase of imbibition, mostly due to protrusion of the
radicle tip and its growth, was highly correlated with the corresponding radicle elongation rate. |
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Keywords: | Additional" target="_blank">Additional Capsicum annuum L internal seed morphology seed swelling |
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