The regulation of glutamate dehydrogenase,nitrite reductase,and nitrate reductase in excised pea roots by nitrite |
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Authors: | J Sahulka |
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Institution: | 1. Department of Plant Physiology, Institute of Experimental Botany of the Czechoslovak Academy of Sciences, Flemingovo nám. 2, 160 00, Praha 6, Czechoslovakia
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Abstract: | Both nitrite reductase and nitrate reductase were induced by nitrite, but there were differences in the time course of induction and in the response to different NO2 - concentrations between these enzymes. NH4 + depressed the induction of nitrite reductase. NADH2 dependent glutamate dehydrogenase activity was enhanced by those NO2-concentrations in the medium at which unmetabolized NO2 - occurred in the roots. NADPH2 and NAD+ dependent GDh activities were not affected. In vivo modification and (or) in vivo activation were probably responsible for the increase in NADH2 dependent GDH activity. |
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